greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
test parameters
sample volume: 200-1000 mL bacterial culture
greener alternative category
, Aligned
storage temp.
15-25°C
usage
kit sufficient for 5 preparations
Quality Level
General description
GenElute HP Endotoxin-Free Plasmid Megaprep Kit 可以简单、快速、低成本的为从重组大肠杆菌培养物中分离不含内毒素的质粒DNA。在1.5小时内可从200 mL - 1.0L LB培养基或200 - 600 mL大肠杆菌TB培养基纯化出多达5 mg内毒素水平≤ 0.1 EU/μg高拷贝数质粒DNA。请注意，实际得率取决于所用的菌株、质粒和培养基。
该产品可为包含内毒素敏感细胞的限制性酶切、连接、测序、PCR、转化和转染等下游应用提供优质、不含内毒素的DNA。
该产品可为包含内毒素敏感细胞的限制性酶切、连接、测序、PCR、转化和转染等下游应用提供优质、不含内毒素的DNA。
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
该产品获得高质量、不含内毒素的DNA，得到的DNA可进行诸多下游应用，如限制性酶切、连接、测序、PCR、转化和内毒素敏感细胞的转染等。
GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.
Features and Benefits
- 可纯化出多达5 mg内毒素水平≤0.1 EU/μg高拷贝数质粒DNA
- 从沉淀细胞到获得纯化的质粒，仅需90分钟
- 采用真空处理形式，无需进行乙醇沉淀
Other Notes
更多信息，请见www.sigma-aldrich.com/genelutehp。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- G3546GenElute™ HP Pro Megaprep Binding Columns 5
- C2112Column Preparation Solution 2
- N7411Neutralization Solution EF
- L1912Lysis Solution
- 2107E-Toxate™ Water, endotoxin, free
- W4639Wash Solution 2
- R1149Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )
查看所有结果 (11)
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
target_organs
Central nervous system, Lungs
存储类别
3 - Flammable liquids
flash_point_f
77.0 °F - closed cup
flash_point_c
25 °C - closed cup
wgk
WGK 3
法规信息
新产品
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Immunogenic Salivary Proteins of Triatoma infestans: Development of a Recombinant Antigen for the Detection of Low-Level Infestation of Triatomines
Alexandra Schwarz
PLoS ONE, e532-e532 (2009)
Functionalization of poly (amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells
Jose Santos
Journal of Controlled Release : Official Journal of the Controlled Release Society (2010)
相关内容
Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.
用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具，用于基于 mRNA 的疫苗和疗法开发。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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