T6791

Trizma ^® 碱

Name: Trizma <SUP>®</SUP> 碱
Brand: SIGMA

BioXtra, pH 10.5-12.0 (1 M in H₂O), ≥99.9% (titration)

别名:

2-氨基-2-(羟甲基)-1,3-丙二醇, THAM, Tris 碱, 三羟甲基氨基甲烷, 氨基丁三醇

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线性分子式:

NH₂C(CH₂OH)₃

化学文摘社编号:

77-86-1

分子量:

121.14

UNSPSC Code:

12352104

NACRES:

NA.25

PubChem Substance ID:

24900405

EC Number:

201-064-4

Beilstein/REAXYS Number:

741883

MDL number:

MFCD00004679

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产品名称

Trizma ^® 碱, BioXtra, pH 10.5-12.0 (1 M in H₂O), ≥99.9% (titration)

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

SMILES string

NC(CO)(CO)CO

description

aminopeptidase substrate

product line

BioXtra

assay

≥99.9% (titration)

form

crystalline

storage condition

dry at room temperature

impurities

≤0.005% Insoluble matter

ign. residue (900°C)

≤0.01% (as SO₄)

loss

≤0.5% loss on drying, 110°C

color

white

pH

10.5-12.0 (1 M in H₂O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

H₂O: 1 M, colorless
water: 678 g/L at 20 °C

anion traces

chloride (Cl^-): ≤0.005%
sulfate (SO₄^2-): ≤0.005%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.020 at 280 in H₂O at 1 M
≤0.025 at 260 in H₂O at 1 M

application(s)

diagnostic assay manufacturing
life science and biopharma
sample preparation

Quality Level

400

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Application

Tris 缓冲液可用于稳定 pH 值，在癌症研究中有助于使用磺基罗丹明 B (SRB) 比色细胞毒性测定法测量细胞毒性。此外，这项研究还利用它来调节培养培养基的 pH 值，以评估代谢途径中糖皮质激素介导的葡萄糖分泌反应。

Features and Benefits

适用于细胞膜渗透性研究以及生物化学和分子生物学研究中的缓冲液制备
通过低重金属污染含量验证测试，确保适用于各种应用
pH 7-9 (25 °C) 范围内有效缓冲，pKa 8.1 (25 °C)
通过痕量阴离子和阳离子检测测试

General description

Tris 碱又称 THAM 或 Trizma^® 碱，作为生物缓冲剂，在各种研究应用中发挥着至关重要的作用。其 pKa 为 8.06，常用于 TAE 和 TBE 缓冲液等缓冲液配方，适合将 pH 值维持在大多数生物体的生理范围内 (pH 7.0-9.0)。但用于蛋白质研究时务必谨慎，因为 Tris 碱可能会干扰某些酶的活性。这种多功能化合物在实验室中不可或缺，可用于制备与生物体液相容的缓冲液，作为标准 pH 溶液，促进乳酸脱氢酶测定、原位杂交和细胞蛋白质提取等方案。此外，Tris 碱在生物化学和分子生物学实验室中也有应用，有助于细胞膜渗透性研究和缓冲液制备。

Other Notes

如需了解生化试剂系列的更多信息，请填写此表。

所有缓冲液的 pH 值都取决于温度和浓度。Tris 缓冲液，温度每降低 1°C，pH 值增加约 0.03 个单位，每稀释十倍 pH 值降低0.03-0.05 个单位。
对于精确的应用，使用经过精心校准的 pH 计和玻璃/甘汞组合电极。

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000517897

0000501629

0000495333

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A rhenium tris-carbonyl derivative has been designed to couple infrared and luminescent detection in cells. Both spectroscopies are consistent with one another; they point out the reliability of the present SCoMPI (for Single Core Multimodal Probe for Imaging) for bimodal

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Trizma ® 碱

BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

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关于此项目

主要文件

属性

产品名称

InChI key

InChI

SMILES string

description

product line

assay

form

storage condition

impurities

ign. residue (900°C)

loss

color

pH

useful pH range

pKa (25 °C)

bp

mp

solubility

anion traces

cation traces

absorption

application(s)

Quality Level

相关类别

说明

Application

Features and Benefits

General description

Other Notes

Legal Information

安全信息

存储类别

wgk

flash_point_f

flash_point_c

ppe

文件

分析证书(COA)

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Trizma ^® 碱

BioXtra, pH 10.5-12.0 (1 M in H₂O), ≥99.9% (titration)