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TRUE5

TrueGel3D 水凝胶试剂盒

Name: TrueGel3D 水凝胶试剂盒
Brand: SIGMA

FAST-PVA, PEG non cell-degradable crosslinker

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关于此项目

NACRES:

NA.71

UNSPSC Code:

12352207

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属性

shipped in

dry ice

storage temp.

−70°C

说明

General description

The gel is formed by stable thioether bonds between maleimide groups of PVA (FAST-PVA) and thiol groups on PEG non cell-degradable crosslinker. Malemide-functionalized PVA is specifically used to decrease the time for gel formation. The PVA polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

Application

TRUE5-1KT is preferred over dextran-based gels for long-term culture of cells which producing carbohydrate degrading enzymes. It is used to culture spheroids which proliferate but do not attach to hydrogel. PVA-PEG hydrogel kits are also used to immobilize cells or specimens for imaging.

Features and Benefits

Fast gelling (takes few seconds to minutes after addition of crosslinker)
Flexibility in modulating gel stiffness
Does not allow cell migration
Cell inert hydrogel (if not customized with RGD peptide)
Cells cannot be recovered

Other Notes

TRUE5-1KT contains FAST-PVA (polymer) in solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

Disclaimer

Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store Mal-PVA in small portions to avoid frequent freeze-thaw cycles.

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pictograms

GHS05

signalword

Warning

hcodes

H290

pcodes

P234 - P390

Hazard Classifications

Met. Corr. 1

flash_point_f

Not applicable

flash_point_c

Not applicable

存储类别

10 - Combustible liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0001615163

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商品

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Utilization of TrueGel3D™ Hydrogels to Study Fibroblast Spreading

Fibroblasts that are present in the stroma secrete glycoproteins and polysaccharides to form extracellular matrix. They maintain the structural integrity within the connective tissue and play a key role in cancer, chronic obstructive pulmonary diseases, idiopathic pulmonary fibrosis, scleroderma and several other diseases.

3D细胞培养水凝胶_细胞培养_TrueGel3D™水凝胶-默克生命科学

TrueGel3D™细胞培养水凝胶是一种由混合聚合物与交联剂混合而成的生化成分限定的水凝胶。与其他基于动物细胞生物提取物（例如小鼠EHS肿瘤细胞）的水凝胶相比，TrueGel3D™细胞培养水凝胶不含任何可能会干扰或造成实验污染的动物源成分。

全球贸易项目编号

货号	GTIN
TRUE5-1KT	04061835539284