主要文件

查看所有文档

XNAT

REDExtract-N-Amp^™ 组织PCR试剂盒

Name: REDExtract-N-Amp<SUP>™</SUP> 组织PCR试剂盒
Brand: SIGMA

sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

别名:

Tissue direct PCR kit, Tissue direct PCR kit with loading dye

登录查看组织和合同定价。

选择尺寸

变更视图

关于此项目

UNSPSC Code:

41106303

EC Number:

254-457-8

NACRES:

NA.55

技术服务

需要帮助？我们经验丰富的科学家团队随时乐意为您服务。

让我们为您提供帮助

Quality Level

200

usage

sufficient for 10 reactions, 10 reactions sufficient for 10 amplifications, 10 reactions sufficient for 10 extractions, sufficient for 100 reactions, 100 reactions sufficient for 100 amplifications, 100 reactions sufficient for 100 extractions, sufficient for 1000 reactions, 1000 reactions sufficient for 1000 amplifications, 1000 reactions sufficient for 1000 extractions

feature

dNTPs included, hotstart, Direct PCR

technique(s)

PCR: suitable

color

red

input

crude DNA

shipped in

wet ice

storage temp.

−20°C

General description

REDExtract-N-Amp^™植物PCR试剂盒包含从鼠尾和其它动物组织、口腔拭子、毛干和唾液中快速提取和扩增基因组DNA所需的所有试剂。无需机械破碎、有机提取、柱纯化或DNA沉淀。该试剂盒还包括专门配制的热启动PCR ReadyMix^™，可直接从提取物中扩增。该配方使用基于抗体的热启动，用于特异性扩增。REDExtract-N-Amp 组织 PCR 试剂盒中含有一种示踪染料，可方便地将PCR反应产物直接上样到琼脂糖凝胶上进行分析。

Application

REDExtract-N-Amp^™ 组织PCR试剂盒已被用于DNA提取和聚合酶链反应（PCR）。

Biochem/physiol Actions

Extract-N-Amp 组织PCR试剂盒用于从多种细胞和组织中快速提取DNA并通过PCR反应扩增感兴趣的目标基因。其采用一种新型提取方法，无需进行耗时的酶促降解或均质化反应。该试剂盒同时包含一种专门配制的热启动PCR ReadyMix^™，可从提取物直接进行扩增。REDExtract-N-Amp PCR ReadyMix含有可作为示踪染料的惰性染料，使得PCR反应物可以方便地上样到琼脂糖凝胶上进行分析。

该试剂盒包含从小鼠尾、毛发、动物组织、唾液和口腔拭子中提取和扩增基因组DNA的经验证实验方案。在常规操作步骤中，基因组提取已在室温下的Tissue Preparation Solution 和Extraction Solution中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和REDExtract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。

该试剂盒包含从小鼠尾、毛发、动物组织、唾液和口腔拭子中提取和扩增基因组DNA的经验证实验方案。在常规操作步骤中，基因组DNA提取自在室温下的组织制备溶液和提取溶液中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和REDExtract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。

Features and Benefits

用于基因分型的快速基因组 DNA 分离的理想选择
在 15 分钟内一步提取用于 PCR 的基因组 DNA
PCR ReadyMix，为提取物直接扩增而专门配制
用于基因组 DNA 高特异性 PCR 扩增的热启动抗体
提取物在4^oC下可稳定保存至少 6 个月。
REDExtract-N-Amp^™含有一种示踪染料，可将PCR反应产物方便地直接上样到琼脂糖凝胶上进行分析。

Other Notes

REDExtract-N-Amp组织PCR试剂盒为从各种细胞和组织中快速提取DNA以及通过PCR扩增靶标提供了所有必需试剂。这种新型提取方法无需长时间的酶消化或均质化处理。该试剂盒还包括特殊配方的热启动PCR反应混合物，可直接从提取物中扩增。 REDExtract-N-Amp PCR ReadyMix含有惰性染料，可作为追踪染料并方便地将PCR反应产物上样到琼脂糖凝胶上进行分析。

更多信息，请见www.sigma-aldrich.com/extract-n-amp。

试剂盒含有Tissue Preparation Solution、Extraction Solution和REDExtract-N-Amp PCR Reaction Mix。

Legal Information

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

pictograms

GHS08,GHS07,GHS09

signalword

Danger

hcodes

H315,H317,H319,H334,H335,H411

pcodes

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

法规信息

低风险生物材料

常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000492040

0000415270

0000415269

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Cisplatin-induced acute renal failure is associated with an increase in the cytokines interleukin (IL)-1beta, IL-18, IL-6, and neutrophil infiltration in the kidney.

Sarah Faubel et al.

The Journal of pharmacology and experimental therapeutics, 322(1), 8-15 (2007-04-03)

We have demonstrated that caspase-1-deficient (caspase-1(-/-)) mice are functionally and histologically protected against cisplatin-induced acute renal failure (ARF). Caspase-1 exerts proinflammatory effects via the cytokines interleukin (IL)-1beta, IL-18, IL-6, and neutrophil recruitment. We sought to determine the role of the

Phylogenetic analyses of cyclidiids (Protista, Ciliophora, Scuticociliatia) based on multiple genes suggest their close relationship with thigmotrichids.

Feng Gao et al.

Molecular phylogenetics and evolution, 75, 219-226 (2014-02-18)

Cyclidiids and thigmotrichids are two diverse groups of scuticociliates, a diverse clade of ciliates that is often difficult to investigate due to the small size and conserved morphology among its members. Compared to other groups (e.g. hypotrichs and oligotrichs), the

Gender-Specific Beneficial Effects of Docosahexaenoic Acid Dietary Supplementation in G93A-SOD1 Amyotrophic Lateral Sclerosis Mice.

Pascual Torres et al.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 17(1), 269-281 (2019-11-23)

Docosahexaenoic acid (DHA) is an essential fatty acid modulating key nervous system functions, including neuroinflammation, and regulation of pre- and postsynaptic membrane formation. DHA concentration decreases in the lumbar spinal cord (LSC) of amyotrophic lateral sclerosis (ALS) patients and murine

Auditory neuroanatomy: a sound foundation for sound processing.

Miguel A Merchán et al.

Frontiers in neuroanatomy, 6, 48-48 (2012-12-12)

Honey bees avoid nectar colonized by three bacterial species, but not by a yeast species, isolated from the bee gut.

Ashley P Good et al.

PloS one, 9(1), e86494-e86494 (2014-01-28)

The gut microflora of the honey bee, Apis mellifera, is receiving increasing attention as a potential determinant of the bees' health and their efficacy as pollinators. Studies have focused primarily on the microbial taxa that appear numerically dominant in the

查看所有相关文献

商品

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Extract-N-Amp™ Tissue Feature

Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.

查看所有结果

实验方案

Extract-N-Amp™ Tissue PCR Kit Protocol

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

REDExtract-N-Amp™ Tissue PCR Kit Protocol

Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.

Extract-N-Amp™ FFPE Tissue PCR Protocol

The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.

Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit

The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.