RIP
Imprint® RNA免疫沉淀试剂盒
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
别名:
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
General description
RNA免疫沉淀（RIP）是研究核糖核蛋白（RNP）复合物中RNA结合蛋白（RBP）及其RNA靶点的强大方法。抗特定RBP的抗体被用于共沉淀RNP，即RBP及其RNA伴侣。然后，可以通过下一代测序鉴定RNA或通过RT-PCR检测特定RNA。
Application
Imprint® RNA免疫沉淀试剂盒已可用于：
- 适用于下游应用
- 从个体靶标表征到全基因组图谱分析技术
- 信号转导途径的表征
- 验证ChIp-chIP和ChIP-seq数据
Features and Benefits
- 更高结合能力的Xtra蛋白A磁珠
- 细胞裂解和免疫沉淀的分步实验方案
- 包含RNA酶抑制剂和无RNA酶试剂，以保持RNA的完整性
- 内附阴性对照，且包含抗体
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
- R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μL
存储类别
3 - Flammable liquids
wgk
WGK 3
flash_point_f
100.4 °F
flash_point_c
38 °C
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
法规信息
低风险生物材料
常规特殊物品
此项目有
Yu-Yuan Qian et al.
Oncotarget, 8(64), 107859-107869 (2018-01-04)
Long non-coding RNA PTENP1, the pseudogene of PTEN tumor suppressor, was previously reported to be a tumour suppressor in some cancer types. However, the precise effects mediated by PTENP1 transcripts within intricate regulatory networks involving molecular interactions with PTEN and
Lin Cao et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 93, 570-579 (2017-07-08)
Non-small cell lung cancer (NSCLC), known as the most common type of lung cancer, has caused great economic losses and brought the patients with NSCLC great suffering. The NSCLC chemo-resistance to cisplatin (DDP) -based chemotherapy remains a huge challenge. The
Huijuan Xiao et al.
Cell & bioscience, 8, 23-23 (2018-03-29)
The acquisition of drug resistance has been considered as a main obstacle for cancer chemotherapy. Tumor protein 53 target gene 1 (TP53TG1), a p53-induced lncRNA, plays a vital role in the progression of human cancers. However, little is known about
Qingling Yuan et al.
The international journal of biochemistry & cell biology, 98, 1-9 (2018-02-24)
Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy. Besides, increasing evidence has demonstrated that long non-coding RNA (lncRNA) HOTTIP played a crucial role in cancer pathogenesis. MiR-637-mediated Akt1 was involved in cell growth, invasion and migration in various
Zhi-Jun Zhu et al.
Biochemical and biophysical research communications, 497(4), 971-977 (2018-02-11)
Long non-coding RNAs are critically involved in oncogenesis in various cancer types. Here we reported a novel lncRNA signature correlated with progression of non-small cell lung cancer (NSCLC). In particular, we identified elevated expression of terminal differentiation-induced noncoding RNA (TINCR)
商品
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Sigma Imprint RNA 免疫沉淀试剂盒用于从 HeLa 细胞中共纯化人 argonaute 2 (Ago2) 相关 RNA。MicroRNA通过Mysticq 试剂进行逆转录和定量。
实验方案
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持