Merck
CN

UPS2

Sigma-Aldrich

蛋白质组学动态范围标准品套装

Protein Mass Spectrometry Calibration Standard

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EC 号:
NACRES:
NA.24

生物来源

human

质量水平

形式

ready-to-use solution

质量

Protein Mass Spectrometry Calibration Standard

浓度

10.6 μg/ampule protein

技术

mass spectrometry (MS): suitable

运输

wet ice

储存温度

−20°C

一般描述

蛋白质组学动态范围标准品套装由48种单独的人源或人序列重组蛋白的混合物产生,每种重组蛋白都被选择用于限制异源翻译后修饰(PTM)。蛋白质标准品由6种蛋白质的6种混合物配制而成,其动态范围为5个数量级,范围为50皮摩尔至500摩尔。在配制之前,通过氨基酸分析(AAA)对每种蛋白质进行定量。

应用

蛋白组学动态范围标准套件用于基于强度的绝对定量(iBAQ),适用 大肠杆菌 蛋白、 胚胎干细胞(ESC)和神经元前体细胞(NPC)蛋白组。 也作为标准品,无标记定量刺突 HeLa 细胞。
蛋白质组学动态范围标准品套装可用于在分析复杂蛋白质样品之前标准化和/或评估质谱(例如,LC-MS / MS,MALDI-TOF-MS等)和电泳分析条件。UPS2 可用于在已知复杂标准样品的运行之间交叉包含宝贵的实验数据集。从而确认分析方法的耐用性和所用仪器的稳定性。此外,生成或比较来自定义不明确的样品的质谱数据的实验室可以使用UPS2作为外部参考,以协助评估结果和实验方法。
蛋白质组学动态范围标准品套装已用于在 Orbitrap 分析仪上使用全离子碎片对动态范围通用蛋白质标准品进行定量。它已被用作LC-MS(液相色谱 - 质谱)/ MS分析中基于强度的蛋白质绝对定量(iBAQ)的标准。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg化学品安全说明书

警示用语:

Danger

危险分类

Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品--人源产品

分析证书(COA)

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示例

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Rosemary Yu et al.
Nature communications, 11(1), 1881-1881 (2020-04-22)
Cells maintain reserves in their metabolic and translational capacities as a strategy to quickly respond to changing environments. Here we quantify these reserves by stepwise reducing nitrogen availability in yeast steady-state chemostat cultures, imposing severe restrictions on total cellular protein and
Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A, et al.
Molecular and Cellular Proteomics, 13, 1925-1925 (2014)
PANDA: A comprehensive and flexible tool for quantitative proteomics data analysis
Chang C, et al.
Bioinformatics, 35, 898-900 (2018)
Lee Dicker et al.
Molecular & cellular proteomics : MCP, 9(12), 2704-2718 (2010-09-09)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics provides a wealth of information about proteins present in biological samples. In bottom-up LC-MS/MS-based proteomics, proteins are enzymatically digested into peptides prior to query by LC-MS/MS. Thus, the information directly available from the LC-MS/MS
Andrew F Jarnuczak et al.
Journal of proteome research, 15(9), 2945-2959 (2016-07-28)
Quantitative mass spectrometry-based proteomics of complex biological samples remains challenging in part due to the variability and charge competition arising during electrospray ionization (ESI) of peptides and the subsequent transfer and detection of ions. These issues preclude direct quantification from

商品

The era of high-throughput proteomics has recently blossomed due in large part to advances in the methods by which proteins and proteomes are analyzed. Improved fractionation techniques, combined with advances in mass spectrometry, have decreased concerns of sample complexity, and directed more focus towards high-throughput techniques.

相关内容

Standardize Your Research. We offer both the Universal Proteomics Standard and the Proteomics Dynamic range Standard as complex, well-defined, well characterized reference standards for mass spectrometry.

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