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Protein quantitation
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facet applications:Protein quantitation
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Duolink® 原位精简操作指南 - 荧光
本页详细介绍了Duolink®原位精简实验方案
Warburg-Christian法测定蛋白质
在测定蛋白质含量时，Warburg-Christian法指的是在280 nm处利用分光光度计对蛋白质样品进行测量。
Duolink® PLA明场实验方案
使用明场显微镜和Duolink® PLA试剂用于检测和定量组织和细胞样品中的个体蛋白质、蛋白质修饰和蛋白质相互作用。
Duolink® PLA Probemaker指南
用于免疫荧光或明场检测的定制PLA探针的创建指南。
Duolink In Situ后复染实验方案
我们建议在Duolink In Situ 荧光用户手册的第7.3节第5步完成扩增步骤后进行复染操作。
通过Duolink®邻位连接技术研究蛋白质互作
上方视频介绍了如何在单次实验中通过简单直接的方法检测、定量和胞内定位蛋白质互作及其修饰情况。
Duolink® PLA多色检测方案
使用荧光显微镜和多色试剂同时检测、可视化和定量一个组织或细胞样本中多达4种的蛋白、蛋白修饰或蛋白互作。
Duolink®PLA流式检测方案
本实验方案描述如何使用Duolink® PLA邻位连接试剂，通过流式细胞术检测细胞群内的单个蛋白质、蛋白修饰和蛋白相互。
二辛可宁酸（BCA）法测定蛋白质
用BCA法定量测定溶液总蛋白浓度的标准程序。
Duolink In Situ后复染实验方案
我们建议在Duolink In Situ 荧光用户手册的第7.3节第5步完成扩增步骤后进行复染操作。
如何创建PLA®探针
Duolink®试剂盒使用原位PLA®技术（一种邻位连接检测技术）准确、客观地量化单个蛋白质，以及这些蛋白在未修饰细胞和组织中的互作和修饰。
Duolink® PLA Probemaker指南
用于免疫荧光或明场检测的定制PLA探针的创建指南。
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Protein Determination by the Bicinchoninic Acid (BCA) Method
A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Production of Dual-labelled Proteins
Refinement of protein dual-labeling protocol using EnPresso® B Defined Nitrogen-free showcased by Vernalis, Ltd. for drug discovery.
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Protein Determination using 2-D Quant Kit
2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.
Protein Determination Modified Lowry Method
To standardize a procedure for the determination of protein by modified Lowry.
Creating PLA® Probes
Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.
Duolink® PLA Brightfield Protocol
Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.
Protein Determination by the Warburg-Christian Method
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.