Protein quantitation

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facet applications:Protein quantitation

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Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.

Duolink® Proximity Ligation Assay Instructional Video

This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.

How to Optimize Duolink® PLA for Flow Cytometry Detection

Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.

Troubleshooting Guide for Duolink® PLA for Flow Cytometry Detection

General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.

Duolink® PLA Flow Cytometry Kits

Duolink® PLA kit enhances flow cytometry for detecting protein interactions accurately.

Duolink® PLA Technology for Neuroscience Applications

Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.

Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection

Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.

EGFR-Family Receptor Dimerization Studies

Investigate EGFR and HER2 dimerization in EGFR-family using cell lines and colon tissues as model systems.

Protein-protein Interactions Detection with PLA

Proteins interact with various molecules, including other proteins, to fulfill complex cellular functions within biological systems.

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Peptide Modifications: N-Terminal, Internal, and C-Terminal

Absolute Quantification of Serum Proteome

Protein-AQUA™™ offers robust targeted proteomics for quantitative studies across diverse research areas.

Multiplex Quantification of Infliximab & Adalimumab

Monoclonal antibodies Infliximab and Adalimumab target TNFα for treating rheumatoid arthritis and autoimmune diseases.

Quantification of Host Cell Proteins

Residual presence of host cell proteins (HCPs) in recombinant therapeutic products has considerable clinical safety risks associated with a potential immunological response in patients.

Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard

Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.

Peptide Sample Amount Determination

Determining the amount of desired product in a peptide sample.

Handling Storage

Handling & Storage of Custom Peptides

Direct Detect® Infrared Spectrometer for Total Protein Quantitation

Learn how total protein quantitation with an infrared spectrometer may be a more accurate and universal alternative to Bradford, BCA, or UV-based methods.

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Single Molecule Detection with Atto 647N NTA

Significant improvement of single molecule tracking by using superior fluorescent NTA-Atto 647N conjugate

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

蛋白定量

蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Protein Determination by the Bicinchoninic Acid (BCA) Method

A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.

Protein Determination using 2-D Quant Kit

2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.

Mass Spectrometry

What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

Creating PLA® Probes

Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

Duolink® PLA Brightfield Protocol

Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.

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