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Protein quantitation
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facet applications:Protein quantitation
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Duolink® PLA邻位连接技术在神经科学中的应用
Duolink®邻位连接技术用于研究神经元相互作用,促进神经科学研究。
Duolink®邻位连接检测操作视频
本视频将逐步向您展示如何使用已固定和透化的玻片细胞进行Duolink® PLA邻位检测实验。
利用in situ PLA检测蛋白质间的互作
蛋白质是维持细胞结构和功能所必需的复杂生物分子。大多数蛋白质通常与各种分子(包括其他蛋白质)互作而发挥功能。
如何优化 Duolink® 邻位连接实验
正确地准备、设置和执行 Duolink® PLA 实验方案需要考虑的因素。
肽修饰:N-末端、内部、和C-末端
肽修饰:N-末端、内部、和C-末端
Duolink® 原位精简操作指南 - 荧光
本页详细介绍了Duolink®原位精简实验方案
Warburg-Christian法测定蛋白质
在测定蛋白质含量时,Warburg-Christian法指的是在280 nm处利用分光光度计对蛋白质样品进行测量。
Duolink® PLA明场实验方案
使用明场显微镜和Duolink® PLA试剂用于检测和定量组织和细胞样品中的个体蛋白质、蛋白质修饰和蛋白质相互作用。
Duolink® PLA Probemaker指南
用于免疫荧光或明场检测的定制PLA探针的创建指南。
蛋白定量
蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。
Duolink® PLA流式蛋白检测试剂盒
了解有关将邻位连接技术 (PLA) 与流式细胞术相结合的新试剂盒的更多信息,实现了蛋白互作流式分析。
通过Duolink®邻位连接技术研究蛋白质互作
上方视频介绍了如何在单次实验中通过简单直接的方法检测、定量和胞内定位蛋白质互作及其修饰情况。
Duolink®邻位连接技术(PLA)多色试剂盒在灵敏的多重蛋白检测中的应用
Duolink® PLA多色技术以及固定组织和细胞中的蛋白互作的定量
Duolink® PLA多色检测方案
使用荧光显微镜和多色试剂同时检测、可视化和定量一个组织或细胞样本中多达4种的蛋白、蛋白修饰或蛋白互作。
Duolink®PLA流式检测方案
本实验方案描述如何使用Duolink® PLA邻位连接试剂,通过流式细胞术检测细胞群内的单个蛋白质、蛋白修饰和蛋白相互。
二辛可宁酸(BCA)法测定蛋白质
用BCA法定量测定溶液总蛋白浓度的标准程序。
Duolink In Situ后复染实验方案
我们建议在Duolink In Situ 荧光用户手册的第7.3节第5步完成扩增步骤后进行复染操作。
如何创建PLA®探针
Duolink®试剂盒使用原位PLA®技术(一种邻位连接检测技术)准确、客观地量化单个蛋白质,以及这些蛋白在未修饰细胞和组织中的互作和修饰。
Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
Duolink® Proximity Ligation Assay Instructional Video
This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.
Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
Industry Guidelines for Multiplex Assay Selection
Get guidance on selecting the right multiplex biomarker immunoassay for your research.
Custom Services Utilizing SMC® Technology
How Single Molecule Counting (SMC®) technology enables quantification of low concentration biomarkers for improved stratification of sample populations, drug development and new biomarker discovery.
Direct Detect® Infrared Spectrometer for Total Protein Quantitation
Learn how total protein quantitation with an infrared spectrometer may be a more accurate and universal alternative to Bradford, BCA, or UV-based methods.
Protein Determination by the Bicinchoninic Acid (BCA) Method
A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
How to Optimize the Duolink® Proximity Ligation Assay
Things to consider for preparation, setup and execution of the Duolink® assay protocol
Immune Responses Associated with Losartan-TKI Combination Immunotherapy in Dogs with Metastatic Osteosarcoma
In this webinar, Dr. Regan will present data regarding a novel immunotherapy in dogs with spontaneous metastatic osteosarcoma. This work led to clinical trials in pediatric and young adult humans with osteosarcoma.
For Research Use Only. Not For Use In Diagnostic
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