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C9422

Sigma-Aldrich

Cellulase from Trichoderma viride

greener alternative

crude powder, 3-10 units/mg solid

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Synonym(s):
1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase
CAS Number:
9012-54-8
Enzyme Commission number:
3.2.1.4 (BRENDA, IUBMB)
EC Number:
232-734-4
MDL number:
MFCD00081510

form

crude powder

specific activity

3-10 units/mg solid

greener alternative product characteristics

Waste Prevention: Greener alternative product characteristics
Learn more about the Principles of Green Chemistry.

greener alternative category

Enabling

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Application

1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase, or cellulase, has been used in a study to examine supra-molecular structural features of cellulose during enzymatic hydrolysis using CP/MAS 13C NMR spectra in combination with line-fitting analysis.

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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Regulatory Information

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

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  5. Does Product C9422, Cellulase from Trichoderma viride, contain any sugars as a stabilizer?

    Product C9422, Cellulase from Trichoderma viride,contains 10 - 20% dextrin as a stabilizer.

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Jack P Wang et al.
Nature communications, 9(1), 1579-1579 (2018-04-22)
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels. Lignin is polymerized from three monomers (monolignols) produced by a grid-like pathway. The pathway in wood formation of Populus trichocarpa
Yunqiao Pu et al.
Carbohydrate research, 341(5), 591-597 (2006-01-31)
Fully bleached softwood kraft pulps were hydrolyzed with cellulase (1,4-(1,3:1,4)-beta-D-glucan 4-glucano-hydrolase, EC 3.2.1.4) from Trichoderma reesei. Supra-molecular structural features of cellulose during enzymatic hydrolysis were examined by using CP/MAS 13C NMR spectra in combination with line-fitting analysis. Different types of
Eliana N Fissore et al.
Journal of agricultural and food chemistry, 58(6), 3793-3800 (2010-02-25)
Chemical and rheological characteristics of fractions enriched in soluble dietary fiber are reported. These fractions were obtained through acid hydrolysis of butternut (Cucurbita moschata Duch ex Poiret) and red beet (Beta vulgaris L. var. conditiva) cell wall enriched powders. Hydrolysis
Mareike Monschein et al.
Bioresource technology, 128, 679-687 (2012-12-12)
Marked slow-down of soluble sugar production at low degree of substrate conversion limits the space-time yield of enzymatic hydrolysis of ligno-cellulosic materials. A simple set of kinetic descriptors was developed to compare reducing sugar release from pure crystalline cellulose (Avicel)
Aditya Bhalla et al.
Bioresource technology, 128, 751-759 (2012-12-19)
Second-generation feedstock, especially nonfood lignocellulosic biomass is a potential source for biofuel production. Cost-intensive physical, chemical, biological pretreatment operations and slow enzymatic hydrolysis make the overall process of lignocellulosic conversion into biofuels less economical than available fossil fuels. Lignocellulose conversions
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