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Merck
CN

C9422

Cellulase from Trichoderma viride

greener alternative

crude powder, 3-10 units/mg solid

Synonym(s):

1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
EC Number:
232-734-4
MDL number:
EC Number:
eCl@ss:
32160410
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form

crude powder

specific activity

3-10 units/mg solid

greener alternative product characteristics

Waste Prevention: Greener alternative product characteristics
Learn more about the Principles of Green Chemistry.

greener alternative category

storage temp.

2-8°C

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Application

1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase, or cellulase, has been used in a study to examine supra-molecular structural features of cellulose during enzymatic hydrolysis using CP/MAS 13C NMR spectra in combination with line-fitting analysis.

Other Notes

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

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Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

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Yunqiao Pu et al.
Carbohydrate research, 341(5), 591-597 (2006-01-31)
Fully bleached softwood kraft pulps were hydrolyzed with cellulase (1,4-(1,3:1,4)-beta-D-glucan 4-glucano-hydrolase, EC 3.2.1.4) from Trichoderma reesei. Supra-molecular structural features of cellulose during enzymatic hydrolysis were examined by using CP/MAS 13C NMR spectra in combination with line-fitting analysis. Different types of
Jack P Wang et al.
Nature communications, 9(1), 1579-1579 (2018-04-22)
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels. Lignin is polymerized from three monomers (monolignols) produced by a grid-like pathway. The pathway in wood formation of Populus trichocarpa
Eliana N Fissore et al.
Journal of agricultural and food chemistry, 58(6), 3793-3800 (2010-02-25)
Chemical and rheological characteristics of fractions enriched in soluble dietary fiber are reported. These fractions were obtained through acid hydrolysis of butternut (Cucurbita moschata Duch ex Poiret) and red beet (Beta vulgaris L. var. conditiva) cell wall enriched powders. Hydrolysis
Antonella Amore et al.
Microbial cell factories, 11, 164-164 (2012-12-27)
The use of lignocellulosic materials for second generation ethanol production would give several advantages such as minimizing the conflict between land use for food and fuel production, providing less expensive raw materials than conventional agricultural feedstock, allowing lower greenhouse gas
Guosheng Xie et al.
PloS one, 8(1), e50171-e50171 (2013-01-12)
Plant glycoside hydrolase family 9 (GH9) comprises typical endo-β-1,4-glucanase (EGases, EC3.2.1.4). Although GH9A (KORRIGAN) family genes have been reported to be involved in cellulose biosynthesis in plants, much remains unknown about other GH9 subclasses. In this study, we observed a

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