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D7295

Deoxynucleotide Mix, 10 mM

Name: Deoxynucleotide Mix, 10 mM
Brand: SIGMA

Molecular Biology Reagent

Synonym(s):

Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs

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About This Item

NACRES:

NA.52

UNSPSC Code:

41106305

Assay:

≥99%

Biological source:

synthetic (organic)

Form:

liquid

Solubility:

H₂O: soluble at 20 °C

Storage temp.:

−20°C

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biological source

synthetic (organic)

Quality Level

300

assay

≥99%

form

liquid

concentration

10 mM

color

colorless

solubility

H₂O: soluble at 20 °C

application(s)

agriculture

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

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General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

Application

dNTP Mix has been used:

in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
in reverse transcription of total RNA to cDNA.
as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
routine and long PCR
manual and automated DNA sequencing
cDNA synthesis and labeling reactions

Features and Benefits

Purity of each dNTP: Minimum 99%
Conveniently formulated; 1 μL is used per 50 μL PCR
Equimolar amounts of each dNTP means less pipetting
Minimize risk of contamination in PCR
UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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The atypical cyclin CNTD2 promotes colon cancer cell proliferation and migration.

Abril Sánchez-Botet et al.

Scientific reports, 8(1), 11797-11797 (2018-08-09)

Colorectal cancer (CRC) is one of the most common cancers worldwide, with 8-10% of these tumours presenting a BRAF (V600E) mutation. Cyclins are known oncogenes deregulated in many cancers, but the role of the new subfamily of atypical cyclins remains

Effect of shenyan xiaobai granule on nephrin and podocin of adriamycin-induced renal injury: A randomised controlled trial.

Shangmei Cao et al.

Journal of ethnopharmacology, 244, 112104-112104 (2019-08-09)

ShenYanXiaoBai granules is a traditional Chinese herbal medicine, It is used widely for the treatment of proteinuria caused by various kidney diseases. This study investigated the mechanism of Shenyan Xiaobai Granule in the treatment of nephritis proteinuria. 100 male wistar

Novel germline BRCA1 and BRCA2 mutations in Turkish women with breast and/or ovarian cancer and their relatives.

Unal Egeli et al.

Cancer investigation, 24(5), 484-491 (2006-08-31)

BRCA1 and BRCA2 gene mutations in patients with breast and/or ovarian cancer have been not characterized in the Turkish population until now. A total of 87 female subjects from two sets of families (38 families total) provided blood samples from

Cyst Fluid Biosignature to Predict Intraductal Papillary Mucinous Neoplasms of the Pancreas with High Malignant Potential.

Ajay V Maker et al.

Journal of the American College of Surgeons, 228(5), 721-729 (2019-02-23)

Current standard-of-care technologies, such as imaging and cyst fluid analysis, are unable to consistently distinguish intraductal papillary mucinous neoplasms (IPMNs) of the pancreas at high risk of pancreatic cancer from low-risk IPMNs. The objective was to create a single-platform assay

The Field Cricket Gryllus assimilis and Two New Sister Species (Orthoptera: Gryllidae)

Weissman, et al.

Annals of the Entomological Society of America, 102, 367-380 (2009)

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Global Trade Item Number

SKU	GTIN
D7295-5ML	04061838104953
D7295-.5ML	04061838104946
D7295-.2ML	04061838104939
D7295-.25ML	04061838056863
D7295-20X.2ML	04061838087355

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