D7295
Deoxynucleotide Mix, 10 mM
Molecular Biology Reagent
Quality Level
form
liquid
concentration
10 mM
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
shipped in
dry ice
storage temp.
−20°C
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General description
Application
- in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
- in reverse transcription of total RNA to cDNA.
- as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
- routine and long PCR
- manual and automated DNA sequencing
- cDNA synthesis and labeling reactions
Features and Benefits
- Purity of each dNTP: Minimum 99%
- Conveniently formulated; 1 μL is used per 50 μL PCR
- Equimolar amounts of each dNTP means less pipetting
- Minimize risk of contamination in PCR
- UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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What are the components to Product D7295, Deoxynucleotide Mix?
The D7295 contains 10mM of each dATP, dCTP, dGTP and TTP in molecular biology grade water. This is for a total concentration of 40 mM.
Does Product No. D7295, Deoxynucleotide Mix contain dTTP or TTP?
The product contains Thymidine 5´-triphosphate sodium salt. Thymidine has a deoxy sugar, so it is a deoxythymidine.
How much Product D7295, Deoxynucleotide Mix, is needed for a PCR reaction?
One μl is sufficient for a standard 50 μl PCR reaction.
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Protocols
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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