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Merck
CN

114774

3′,3′′,5′,5′′-Tetrabromophenolphthalein ethyl ester potassium salt

indicator grade

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About This Item

Empirical Formula (Hill Notation):
C22H13Br4KO4
CAS Number:
62637-91-6
Molecular Weight:
700.05
EC Number:
263-661-6
UNSPSC Code:
12171500
PubChem Substance ID:
24847193
Beilstein/REAXYS Number:
3848263
MDL number:
MFCD00011662

Key Documents

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InChI key

WCIQBKUTYDIBJC-UHFFFAOYSA-M

InChI

1S/C22H14Br4O4.K/c1-2-30-22(29)14-6-4-3-5-13(14)19(11-7-15(23)20(27)16(24)8-11)12-9-17(25)21(28)18(26)10-12;/h3-10,27H,2H2,1H3;/q;+1/p-1

SMILES string

[K+].CCOC(=O)c1ccccc1\C(c2cc(Br)c([O-])c(Br)c2)=C3\C=C(Br)C(=O)C(Br)=C3

grade

indicator grade

visual transition interval

3.0-3.5, yellow to green, 4.2-4.9, blue-green to blue-violet

mp

210 °C (dec.) (lit.)

λmax

386 nm (2nd), 593 nm

Biochem/physiol Actions

3′,3′′,5′,5′′-Tetrabromophenolphthalein ethyl ester is used in the spectrophotometric method of total protein determination.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000060755
0000060749
0000049804
0000059832
0000043004

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Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods.
Zaia DAM, et al.
Brazilian Archives of Biology and Technology, 48, 385-388 (2005)
H T Chang et al.
Electrophoresis, 22(3), 394-398 (2001-03-22)
Electroelution of protein bands from a gel has advantages over the competitive common technique requiring gel sectioning with respect to yield, speed and the potential for computer-controlled application to multicomponent two-dimensional (2-D) gels. The electroelution design for the commercial high-performance
[Pharmaco-analytical application of tetrabromophenolphthalein ethyl ester indicator dye. III. Interaction between alkaloids and TBPE].
G Szász et al.
Acta pharmaceutica Hungarica, 50(4), 161-165 (1980-07-01)
The application of the TBP test to bile.
E G Saker
Journal of analytical toxicology, 8(1), 51-51 (1984-01-01)
Spectrophotometric determination of histamine in mast cells, muscle and urine by solvent extraction with copper(II) and tetrabromophenolphthalein ethyl ester.
T Sakai et al.
The Analyst, 109(12), 1569-1572 (1984-12-01)
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