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Merck
CN

198285

Sigma-Aldrich

Nigrosin

certified by the BSC

Synonym(s):

Acid black 2, Nigrosin water soluble

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About This Item

CAS Number:
8005-03-6
Colour Index Number:
50420
MDL number:
MFCD00044681
UNSPSC Code:
12171500
NACRES:
NA.47

Key Documents

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Agency

certified by the BSC

Quality Level

200

form

solid

solubility

H2O: 0.1%
H2O: soluble

λmax

570 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Application

Certified for use in Dorner′s spore stain. Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.
Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM1524
MKCJ5622
SHBL0637
MKCG3346
MKCD8042

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of Product 198285, Nigrosin, in water?

    The maximum solubility in water can vary from 5 to 10%, depending upon the lot number.

  6. What is the purity of Product 198285, Nigrosin, Water Soluble?

    No dye purity is determined because the dye is a complex mixture of dyes with an unknown constitution.

  7. What is the molecular weight for Product 198285, Nigrosin?

    No formula weight or empirical formula is available. The dye is innately a mixture of dyes.

  8. Is Product 198285, Nigrosin, soluble in ethanol?

    Solubility is stated to be only 0.0 to 0.4% in ethanol.

  9. Has Product 198285, Nigrosin, been used to test for sperm viability?

    Yes. 10% Nigrosin Water Soluble is often used with 5% aqueous Eosin Y to determine live versus dead sperm. A procedure is given in Kjeldsberg, CR and Knight JA; Body Fluids, Second Edition (ASCP Press, Chicago 1986), p 168.

  10. During the certification testing for Product 198285, Nigrosin, what organism is used for testing?

    The exact organism can vary, but typically a 2-4 day old culture of a rapid spore former such as Bacillus cereus is utilized.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Journal of biomedical optics, 25(9) (2020-09-06)
Photoacoustic imaging (PAI) enables the detection of blood hemoglobin (HB) concentration and oxygenation (sO2) with high contrast and resolution. Despite the heavy use of photoacoustically determined total hemoglobin (THb) and oxygenation (sO2) biomarkers in PAI research, their relationship with underlying
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Journal of biomedical optics, 24(12), 1-11 (2019-10-19)
Photoacoustic tomography (PAT) is intrinsically sensitive to blood oxygen saturation (sO2) in vivo. However, making accurate sO2 measurements without knowledge of tissue- and instrumentation-related correction factors is extremely challenging. We have developed a low-cost flow phantom to facilitate validation of
William M Buckett
Asian journal of andrology, 5(3), 209-212 (2003-08-26)
To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm. Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analysis, eosin-nigrosin (EN) staining and the HOS test. A further EN stain was
Emmanuel I Odongo-Aginya et al.
African health sciences, 7(1), 33-36 (2007-07-03)
The Kato-Katz thick smear technique is the standard technique recommended by the World Health Organisation for the quantitative diagnosis of Schistosoma mansoni and other intestinal helminth infections. The major problem of the technique is that a few hours after the
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