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Sigma-Aldrich

Tetrabromophenol Blue

Dye content 75 %

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Synonym(s):
3,3′,3″,4,5,5′,5″,6-Octabromophenolsulfonphthalein
Empirical Formula (Hill Notation):
C19H6Br8O5S
CAS Number:
4430-25-5
Molecular Weight:
985.54
Beilstein:
378438
EC Number:
224-622-9
MDL number:
MFCD00005876
PubChem Substance ID:
24851906
NACRES:
NA.47

Assay

≥75.0% (HPLC)

Quality Level

200

form

powder

composition

Dye content, 75%

mp

204 °C (dec.) (lit.)

λmax

388 nm (2nd)
605 nm

ε (extinction coefficient)

≥55000 at 606-616 nm in ethanol and water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3c(Br)c(Br)c(Br)c(Br)c23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H6Br8O5S/c20-7-1-5(2-8(21)16(7)28)19(6-3-9(22)17(29)10(23)4-6)11-12(24)13(25)14(26)15(27)18(11)33(30,31)32-19/h1-4,28-29H

InChI key

QPMIVFWZGPTDPN-UHFFFAOYSA-N

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Biochem/physiol Actions

Tetrabromophenol Blue (TBPB) belongs to the group of sulfonephthalein acid-base indicators. It is employed in the potentiometric titration of the halogens with Hg2(NO3)2. In addition, it has been used as a dye indicator in urine dipstick test to detect proteinuria. In this assay, with increasing concentration of urinary protein, TBPB sequentially changes color from pale green to blue. Binding of TBPB to proteins is dependent on pH.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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