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Sigma-Aldrich

Erythrosin B

Dye content ≥95 %

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Synonym(s):
Solvent Red 140
Empirical Formula (Hill Notation):
C20H8I4O5
CAS Number:
15905-32-5
Molecular Weight:
835.89
Colour Index Number:
45430:2
EC Number:
240-046-0
MDL number:
MFCD00005044
PubChem Substance ID:
24851985

form

powder

Quality Level

100

composition

Dye content, ≥95%

color

orange to red-orange

mp

303 °C (dec.) (lit.)

solubility

1 M NH4OH: 1 mg/mL
1 M NH4OH: soluble 1 mg/mL, orange to red

ε (extinction coefficient)

≥102000 at 526-534 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥13000 at 310-318 nm in 1 M NH4OH at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥32000 at 258-266 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(I)cc2c(Oc3c(I)c(O)c(I)cc3C24OC(=O)c5ccccc45)c1I

InChI

1S/C20H8I4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

InChI key

OALHHIHQOFIMEF-UHFFFAOYSA-N

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Application

Erythrosin B has been used as a dye in fluorescence lifetime imaging.

Biochem/physiol Actions

Erythrosin B, also called as eosin B, is a xanthene dye. It belongs to the family of fluorescein dyes. It plays a major role in inhibiting dopamine uptake and high affinity 3H-ouabain binding and ion transport in synaptosomes from rat caudate nucleus.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Bright monomeric red fluorescent protein with an extended
fluorescence lifetime
Merzlyak EM
Nature Methods, 4, 555-557 (2007)
Erythrosin B inhibits dopamine transport in rat caudate synaptosomes.
Lafferman JA and Silbergeld EK
Science, 205, :410-:412 (1979)
Erythrosin B is a specific inhibitor of high affinity 3H-ouabain binding and ion transport in rat brain
Silbergeld E K
Neuropharmacology, 20, 87-90 (1981)
Practical and reliable FRET/FLIM pair of fluorescent proteins.
Shcherbo D
BMC biotechnology, 2009-2009 null
Hamed Gilzad Kohan et al.
Xenobiotica; the fate of foreign compounds in biological systems, 45(7), 642-652 (2015-01-08)
1. Influx and efflux proteins play a major role in the overall uptake and efficacy of chemotherapeutic agents and cellular chemo-resistance. 2. The present study investigated the time course and dose dependency of the induction of three efflux proteins, P-gp
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