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Merck
CN

201545

Fast Blue RR

Synonym(s):

4-Benzoylamino-2,5-dimethoxyaniline, Azoic Diazo No. 24

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About This Item

Empirical Formula (Hill Notation):
C15H16N2O3
CAS Number:
6268-05-9
Molecular Weight:
272.30
PubChem Substance ID:
24852010
UNSPSC Code:
12171500
Colour Index Number:
37155
NACRES:
NA.47
EC Number:
228-441-6
MDL number:
MFCD00008369

Key Documents

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InChI key

DDRCIGNRLHTTIW-UHFFFAOYSA-N

InChI

1S/C15H16N2O3/c1-19-13-9-12(14(20-2)8-11(13)16)17-15(18)10-6-4-3-5-7-10/h3-9H,16H2,1-2H3,(H,17,18)

SMILES string

COc1cc(NC(=O)c2ccccc2)c(OC)cc1N

assay

>96.5% (HPLC)

form

solid

mp

165-168 °C (lit.)

λmax

323 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

Related Categories

Application

Fast Blue RR has been used to detect alkaline phosphatase activity.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appear black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SHBN8352
SHBQ1671
MKCJ2160
MKCC7697
MKBW6837V

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Andrei Mayer et al.
Cerebral cortex (New York, N.Y. : 1991), 29(12), 5098-5115 (2019-03-20)
The posterior parietal cortex (PPC) is a central hub for the primate forebrain networks that control skilled manual behavior, including tool use. Here, we quantified and compared the sources of thalamic input to electrophysiologically-identified hand/forearm-related regions of several PPC areas
Claude Reiss, Simone Parvez, Gilles Labbe
Advances in Molecular Toxicology (1998)
C. F. A. Culling
Handbook of Histopathological and Histochemical Techniques (2013)
The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells.
Chung TH
Biomaterials, 28, 2959-2966 (2007)
Jutta Schade et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 56(2), 147-155 (2007-10-31)
The expression of dipeptidyl peptidase 4 (DP4, CD26) affects T-cell recruitment to lungs in an experimental rat asthma model. Furthermore, the gene of the structural homologous DP10 represents a susceptibility locus for asthma in humans, and the functional homologous DP8/9

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