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Merck
CN

201677

Janus Green B

certified by the BSC, Dye content 65 %

Synonym(s):

3-Diethylamino-7-(4-dimethylaminophenylazo)-5-phenylphenazinium chloride, Diazin Green S, Union Green B

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About This Item

Empirical Formula (Hill Notation):
C30H31ClN6
CAS Number:
2869-83-2
Molecular Weight:
511.06
NACRES:
NA.47
PubChem Substance ID:
24852018
UNSPSC Code:
12171500
Colour Index Number:
11050
EC Number:
220-695-6
MDL number:
MFCD00011758

Key Documents

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Product Name

Janus Green B, certified by the BSC, Dye content 65 %

InChI key

XXACTDWGHQXLGW-UHFFFAOYSA-M

InChI

1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1

SMILES string

[Cl-].CCN(CC)c1ccc2nc3ccc(cc3[n+](-c4ccccc4)c2c1)\N=N\c5ccc(cc5)N(C)C

agency

certified by the BSC

form

powder

composition

Dye content, 65%

technique(s)

microbe id | staining: suitable

color

black
very dark brown
very dark green

λmax

395 nm
660 nm (2nd)

ε (extinction coefficient)

≥28000 at 651-677 nm in ethanol: water (1:1) at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.

Biochem/physiol Actions

Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS9767
SHBS9336
0000242157
0000242157
SHBP9776

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Intracellular insulin quantification by cell-ELISA
Andersen P and Vermette P
Experimental Cell Research, 347, 14-23 (2016)
David E. Sadava
Cell Biology: Organelle Structure and Function (1993)
R. W. Sabnis
Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications (2010)
Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method
Lee BC
Anatomical Record. Part B, New Anatomist, 286, 1-7 (2005)
Telocytes in trachea and lungs
Zheng Y
Journal of Cellular and Molecular Medicine, 15, 2262-2268 (2011)
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