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Merck
CN

202029

Metanil Yellow

Dye content 70 %

Synonym(s):

3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt, Acid Yellow 36

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About This Item

Empirical Formula (Hill Notation):
C18H14N3NaO3S
CAS Number:
587-98-4
Molecular Weight:
375.38
EC Number:
209-608-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24852034
MDL number:
MFCD00007486
Colour Index Number:
13065
Beilstein/REAXYS Number:
3831568

Key Documents

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Product Name

Metanil Yellow, Dye content 70 %

InChI key

NYGZLYXAPMMJTE-ANVLNOONSA-M

InChI

1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;

SMILES string

[Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2

form

solid

composition

Dye content, 70%

technique(s)

titration: suitable

pH

1.5-2.7, red to yellow

λmax

414 nm

ε (extinction coefficient)

≥18500 at 415.0-419.0 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

Biochem/physiol Actions

Metanil yellow is an azo dye. It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast. Metanil yellow changes its color from red at pH 1.2 to yellow at pH 2.3.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 2

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN5979
BCCM4865
BCCF8035
MKCJ4314
BCCC2672

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An Improved Stain for Connective Tissue Cells in Plastic-Embedded Sections
Cannon M S
Laboratory Medicine, 10, 783?785-783?785 (1979)
Photocatalytic degradation of azo dye Metanil Yellow: Optimization and kinetic modeling using a chemometric approach
Sleiman M
Applied Catalysis. B, Environmental, 77, 1-2 (2007)
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et.al.
Biotechnic & Histochemistry, 66, 169-172 (1991)
Cellular Localization of Latent Murine Cytomegalovirus
Koffron A J, et.al.
Journal of Virology, 72, 95-103 (1998)
R. W. Sabnis
Handbook of Acid-Base Indicators (2007)
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