206342
Celestine blue
Dye content 80 %
Synonym(s):
Mordant Blue 14
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About This Item
Empirical Formula (Hill Notation):
C17H18ClN3O4
CAS Number:
Molecular Weight:
363.80
EC Number:
216-346-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
51050
Beilstein/REAXYS Number:
3830911
form
powder
composition
Dye content, 80%
mp
227-230 °C
solubility
water: 0.1%
λmax
642 nm
ε (extinction coefficient)
≥29000 at 640-650 nm in water at 0.01 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)c(O)c(O)cc3C(N)=O
InChI
1S/C17H17N3O4.ClH/c1-3-20(4-2)9-5-6-11-13(7-9)24-16-14(19-11)10(17(18)23)8-12(21)15(16)22;/h5-8H,3-4H2,1-2H3,(H3-,18,19,21,22,23);1H
InChI key
VXAFJUDTZSMEFN-UHFFFAOYSA-N
Application
May be substituted for hematoxylin in hematoxylin and eosin procedures.
Biochem/physiol Actions
Celestine blue (CB) is an oxazine dye and is used in combination with the alum hematoxylin, as a nuclear stain. It gives good nuclear definition and is resistant to strong acid dyes. Celestine blue is commonly used for routine staining of fixed sections. It has been used as a new electroactive indicator in DNA biosensors.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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H Troyer et al.
Stain technology, 56(1), 39-43 (1981-01-01)
A staining procedure is described for use with glycol methacrylate embedded tissue sections which does not stain the plastic embedment or remove the sections from the glass slides. The basic dye is celestine blue B. It is prepared by treating
A Rami et al.
Brain research, 866(1-2), 299-312 (2000-05-29)
The differentiated cells seem to share the ability to induce their own death by the activation of an internally encoded suicide program. When activated, this suicide program initiates a characteristic form of cell death called apoptosis. A central challenge in
M K Dutt
Microscopica acta, 86(3), 201-205 (1982-09-01)
This paper presents methods for specific staining of nuclei with aqueous solutions of celestin blue B and gallocyanine in tissue sections from which RNA has been extracted selectively with concentrated phosphoric acid at 5 degrees C for 20 min or
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 206342-5G | 04061838768612 |
| 206342-25G | 04061838768605 |