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About This Item
Empirical Formula (Hill Notation):
C17H18ClN3O4
CAS Number:
Molecular Weight:
363.80
EC Number:
216-346-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
51050
Beilstein/REAXYS Number:
3830911
form
powder
composition
Dye content, 80% HPLC
mp
227-230 °C
solubility
water: 0.1%
λmax
642 nm
ε (extinction coefficient)
≥29000 at 640-650 nm in water at 0.01 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)c(O)c(O)cc3C(N)=O
InChI
1S/C17H17N3O4.ClH/c1-3-20(4-2)9-5-6-11-13(7-9)24-16-14(19-11)10(17(18)23)8-12(21)15(16)22;/h5-8H,3-4H2,1-2H3,(H3-,18,19,21,22,23);1H
InChI key
VXAFJUDTZSMEFN-UHFFFAOYSA-N
Application
May be substituted for hematoxylin in hematoxylin and eosin procedures.
Biochem/physiol Actions
Celestine blue (CB) is an oxazine dye and is used in combination with the alum hematoxylin, as a nuclear stain. It gives good nuclear definition and is resistant to strong acid dyes. Celestine blue is commonly used for routine staining of fixed sections. It has been used as a new electroactive indicator in DNA biosensors.
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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