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Merck
CN

206342

Celestine blue

Dye content 80 %

Synonym(s):

Mordant Blue 14

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About This Item

Empirical Formula (Hill Notation):
C17H18ClN3O4
CAS Number:
Molecular Weight:
363.80
EC Number:
216-346-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
51050
Beilstein/REAXYS Number:
3830911
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InChI key

VXAFJUDTZSMEFN-UHFFFAOYSA-N

InChI

1S/C17H17N3O4.ClH/c1-3-20(4-2)9-5-6-11-13(7-9)24-16-14(19-11)10(17(18)23)8-12(21)15(16)22;/h5-8H,3-4H2,1-2H3,(H3-,18,19,21,22,23);1H

SMILES string

[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)c(O)c(O)cc3C(N)=O

form

powder

composition

Dye content, 80%

mp

227-230 °C

solubility

water: 0.1%

λmax

642 nm

ε (extinction coefficient)

≥29000 at 640-650 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Application

May be substituted for hematoxylin in hematoxylin and eosin procedures.

Biochem/physiol Actions

Celestine blue (CB) is an oxazine dye and is used in combination with the alum hematoxylin, as a nuclear stain. It gives good nuclear definition and is resistant to strong acid dyes. Celestine blue is commonly used for routine staining of fixed sections. It has been used as a new electroactive indicator in DNA biosensors.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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H Troyer et al.
Stain technology, 56(1), 39-43 (1981-01-01)
A staining procedure is described for use with glycol methacrylate embedded tissue sections which does not stain the plastic embedment or remove the sections from the glass slides. The basic dye is celestine blue B. It is prepared by treating
A Rami et al.
Brain research, 866(1-2), 299-312 (2000-05-29)
The differentiated cells seem to share the ability to induce their own death by the activation of an internally encoded suicide program. When activated, this suicide program initiates a characteristic form of cell death called apoptosis. A central challenge in
Y Kiyota et al.
Experimental brain research, 95(3), 388-396 (1993-01-01)
The objective of this study was to assess whether dimethylthiourea (DMTU), an established free radical scavenger, ameliorates ischaemic damage due to 2-3 h of transient middle cerebral artery (MCA) occlusion, induced by an intraluminal filament. A major point addressed was
Christopher Layton, John D. Bancroft
Bancroft's Theory and Practice of Histological Techniques (2013)
Celestine blue as a new indicator in
electrochemical DNA biosensors
Asl E H
Science China: Chemistry (2015)

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