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Merck
CN

212407

Direct Blue 71

Dye content 50 %

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About This Item

Empirical Formula (Hill Notation):
C40H23N7O13S4 · 4Na
CAS Number:
Molecular Weight:
1029.87
PubChem Substance ID:
UNSPSC Code:
12171500
Colour Index Number:
34140
NACRES:
NA.47
EC Number:
224-531-4
MDL number:
Technical Service
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form

powder

Quality Segment

composition

Dye content, 50%

λmax

594 nm

ε (extinction coefficient)

≥30000 at 582-588 nm in water at 0.01 g/L, ≥15000 at 283-292 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Nc1ccc2c(O)c(N=Nc3ccc(N=Nc4ccc(N=Nc5cc(c6cccc(c6c5)S([O-])(=O)=O)S([O-])(=O)=O)c7ccccc47)c8ccc(cc38)S([O-])(=O)=O)c(cc2c1)S([O-])(=O)=O

InChI

1S/C40H27N7O13S4.4Na/c41-22-8-10-25-21(16-22)17-38(64(58,59)60)39(40(25)48)47-46-35-15-14-34(28-11-9-24(20-30(28)35)61(49,50)51)45-44-33-13-12-32(26-4-1-2-5-27(26)33)43-42-23-18-31-29(37(19-23)63(55,56)57)6-3-7-36(31)62(52,53)54;;;;/h1-20,48H,41H2,(H,49,50,51)(H,52,53,54)(H,55,56,57)(H,58,59,60);;;;/q;4*+1/p-4

InChI key

QTTDXDAWQMDLOF-UHFFFAOYSA-J

General description

Direct blue 71 is an azo dye with three azo bonds. It is useful to stain membrane-immobilized antibodies and proteins for the investigation of antigen-antibody interactions.

Application

Direct blue 71 has been used to stain sliced tissue sections mounted on the PVDF for in situ proteome analysis, and to stain the electroblotted membranes and sections of the brain to visualize the structure. Direct blue staining is also suitable for the assessment of protein binding and qualitative validation of protein concentration.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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