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Merck
CN

212407

Direct Blue 71

Dye content 50 %

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About This Item

Empirical Formula (Hill Notation):
C40H23N7O13S4 · 4Na
CAS Number:
4399-55-7
Molecular Weight:
1029.87
PubChem Substance ID:
24852733
UNSPSC Code:
12171500
Colour Index Number:
34140
NACRES:
NA.47
EC Number:
224-531-4
MDL number:
MFCD00003971

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InChI key

QTTDXDAWQMDLOF-UHFFFAOYSA-J

InChI

1S/C40H27N7O13S4.4Na/c41-22-8-10-25-21(16-22)17-38(64(58,59)60)39(40(25)48)47-46-35-15-14-34(28-11-9-24(20-30(28)35)61(49,50)51)45-44-33-13-12-32(26-4-1-2-5-27(26)33)43-42-23-18-31-29(37(19-23)63(55,56)57)6-3-7-36(31)62(52,53)54;;;;/h1-20,48H,41H2,(H,49,50,51)(H,52,53,54)(H,55,56,57)(H,58,59,60);;;;/q;4*+1/p-4

SMILES string

[Na+].[Na+].[Na+].[Na+].Nc1ccc2c(O)c(N=Nc3ccc(N=Nc4ccc(N=Nc5cc(c6cccc(c6c5)S([O-])(=O)=O)S([O-])(=O)=O)c7ccccc47)c8ccc(cc38)S([O-])(=O)=O)c(cc2c1)S([O-])(=O)=O

form

powder

composition

Dye content, 50%

λmax

594 nm

ε (extinction coefficient)

≥30000 at 582-588 nm in water at 0.01 g/L, ≥15000 at 283-292 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Related Categories

General description

Direct blue 71 is an azo dye with three azo bonds. It is useful to stain membrane-immobilized antibodies and proteins for the investigation of antigen-antibody interactions.

Application

Direct blue 71 has been used to stain sliced tissue sections mounted on the PVDF for in situ proteome analysis, and to stain the electroblotted membranes and sections of the brain to visualize the structure. Direct blue staining is also suitable for the assessment of protein binding and qualitative validation of protein concentration.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT0622
SHBQ1807
SHBM3919
MKCJ3381
MKCB3141V

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