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Merck
CN

219215

Leucocrystal Violet

Synonym(s):

4,4′,4′′-Methylidynetris(N,N-dimethylaniline), Leuco Crystal Violet

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About This Item

Linear Formula:
CH[C6H4N(CH3)2]3
CAS Number:
Molecular Weight:
373.53
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
210-043-9
MDL number:
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InChI key

OAZWDJGLIYNYMU-UHFFFAOYSA-N

InChI

1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3

SMILES string

CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

form

powder

technique(s)

microbe id | staining: suitable

mp

175-177 °C (lit.)

λmax

260 nm

ε (extinction coefficient)

≥700 at 257-263 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

Application

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.

Biochem/physiol Actions

Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.

Disclaimer

Photo-ionization can cause color variations

pictograms

Environment

signalword

Warning

hcodes

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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L G Rushing et al.
Journal of chromatography. B, Biomedical sciences and applications, 688(2), 325-330 (1997-01-24)
A sensitive analytical procedure for the determination of residues of leucomalachite green (LMG)-malachite green (MG) and leucogentian violet (LGV)-gentian violet (GV) in catfish or trout tissue is presented. Frozen (-20 degrees C) fish fillets were cut into small pieces and
D R Doerge et al.
Rapid communications in mass spectrometry : RCM, 10(12), 1479-1484 (1996-01-01)
Gentian violet (GV) is a triphenylmethane dye antiseptic with potential for illegal use in livestock production, especially aquaculture where the related malachite green has been widely used. This potential misuse has regulatory importance because of the observed rodent carcinogenicity of
Detection Method for Histamine-Producing, Dairy-Related
Bacteria using Diamine Oxidase and Leucocrystal Violet
SUMNER S S
Journal of Food Protection, 52, 105-108 (1989)
Craig A. Coppock
Contrast: An Investigator's Basic Reference Guide to Fingerprint Identification Concepts (2007)
Use of leuco crystal violet to enhance shoe prints in blood.
Bodziak WJ
Forensic Science International, 82, 45-52 (1996)

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