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Merck
CN

219215

Leucocrystal Violet

Synonym(s):

4,4′,4′′-Methylidynetris(N,N-dimethylaniline), Leuco Crystal Violet

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About This Item

Linear Formula:
CH[C6H4N(CH3)2]3
CAS Number:
603-48-5
Molecular Weight:
373.53
NACRES:
NA.47
PubChem Substance ID:
24853114
UNSPSC Code:
12171500
EC Number:
210-043-9
MDL number:
MFCD00008314

Key Documents

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InChI key

OAZWDJGLIYNYMU-UHFFFAOYSA-N

InChI

1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3

SMILES string

CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

form

powder

technique(s)

microbe id | staining: suitable

mp

175-177 °C (lit.)

λmax

260 nm

ε (extinction coefficient)

≥700 at 257-263 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Related Categories

Application

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.

Biochem/physiol Actions

Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.

Disclaimer

Photo-ionization can cause color variations

pictograms

Environment
GHS09

signalword

Warning

hcodes

H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000236216
0000236216
MKBW1177V
MKBR8820V
MKBR8440V

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J E Roybal et al.
Journal of chromatography, 467(1), 259-266 (1989-04-21)
High-performance liquid chromatographic conditions are reported for the electrochemical detection (ED) of Gentian Violet, its demethylated metabolites, Leucogentian Violet and Methylene Blue. Gentian Violet, its demethylated metabolites and Leucogentian Violet were separated within 14 min on a cyano column eluted
R T Wilson et al.
Journal of AOAC International, 77(5), 1137-1142 (1994-09-01)
A gas chromatographic/mass spectrometric (GC/MS) procedure for confirming the identity of leucogentian violet (LGV) in chicken fat was developed for regulatory application. The unused portion of the extract remaining from a determinative procedure was back-extracted into an organic phase, concentrated
Xueli Wu et al.
Journal of chromatography. A, 1172(2), 121-126 (2007-10-24)
A method has been developed for the simultaneous determination of malachite green, gentian violet and their leuco-metabolites in various aquatic products using isotope dilution liquid chromatography-linear ion trap mass spectrometry without post-column oxidation. Sample was extracted with McIlvaine buffer and
J J McDonald et al.
Drug metabolism and disposition: the biological fate of chemicals, 12(3), 330-336 (1984-05-01)
Cellular suspensions of microflora isolated from human feces and from intestinal contents of rat and chicken transformed the triphenylmethane dye gentian violet under anaerobic conditions. Pure cultures of nine genera of strict and facultative anaerobes were also active. All pure
P G Schermerhorn et al.
Journal of AOAC International, 77(6), 1454-1460 (1994-11-01)
A laboratory trial was completed for a liquid chromatographic method that can quantitate leucogentian violet (LGV) in chicken fat at 10 ppb. With this method, LGV is isolated from the fat matrix by a series of liquid-liquid extractions. This trial
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