227935
Azure B
prepared by direct synthesis
Synonym(s):
N,N,N′-Trimethylthionin, Azure I, Methyleneazure
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About This Item
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
EC Number:
208-511-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
52010
Beilstein/REAXYS Number:
3922630
Quality Level
assay
>85.0% (HPLC)
form
powder
mp
205-210 °C (dec.) (lit.)
solubility
water: 1 mg/mL, blue to very deep blue
λmax
648 nm
ε (extinction coefficient)
≥13000 at 241-247 nm, ≥36000 at 287-293 nm, ≥67000 at 638-644 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]
InChI
1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H
InChI key
DNDJEIWCTMMZBX-UHFFFAOYSA-N
Application
Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule.
Biochem/physiol Actions
Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localization of nucleic acids and lignin.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Barbara J. Bain, Imelda Bates, Mike A Laffan
Dacie and Lewis Practical Haematology (2016)
A simple, reliable M'Fadyean stain for visualizing the Bacillus anthracis capsule
Owen MP
Journal of Microbiological Methods, 92, 264-269 (2013)
U.S. Forest Service research note FPL (1964)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 227935-5G | 04061838781031 |
| 227935-100G | 04061838781024 |