Merck
CN
All Photos(3)

Documents

302643

Sigma-Aldrich

Trypan Blue

≥80% (HPLC), Dye content 60 %

Sign Into View Organizational & Contract Pricing
All Photos(3)
Synonym(s):
Direct blue 14
Linear Formula:
C34H24N6O14S4Na4
CAS Number:
72-57-1
Molecular Weight:
960.81
Colour Index Number:
23850
Beilstein:
4360496
EC Number:
200-786-7
MDL number:
MFCD00003969
PubChem Substance ID:
24858268
NACRES:
NA.47

Assay

≥80% (HPLC)

form

powder

composition

Dye content, 60%

color

dark green to brown, or Brown-Black

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

λmax

607 nm

ε (extinction coefficient)

≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

human ... PTPN1(5770) , PTPRF(5792)

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.

Application

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:
  • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
  • to stain plant tissue in order to visualize cell death.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Thangaraj Parimelazhagan
Pharmacological Assays of Plant-Based Natural Products (2013)
Ocular toxicity study of trypan blue injected into the vitreous cavity of rabbit eyes
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 239, 698-704 (2001)
Lack of Bax Prevents Influenza A Virus-Induced Apoptosis and Causes Diminished Viral Replication
McLean J E, et.al.
Journal of Virology, 83, 8233-8246 (2009)
Role of caspase-10 in the death of acute leukemia cells
Guo W
Oncology Letters, 12, 1623-1629 (2016)
Jeffrey E McLean et al.
Journal of virology, 83(16), 8233-8246 (2009-06-06)
The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service