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Merck
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364584

Nitrogen-15N2

98 atom % 15N

Synonym(s):

15N Labeled nitrogen

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About This Item

Linear Formula:
15N2
CAS Number:
Molecular Weight:
30.00
NACRES:
NA.12
PubChem Substance ID:
UNSPSC Code:
12141903
MDL number:
Isotopic purity:
98 atom % 15N
Assay:
99% (CP)
Mass shift:
M+2
Form:
gas
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vapor density

1.34 (vs air)

Quality Level

isotopic purity

98 atom % 15N

assay

99% (CP)

form

gas

mass shift

M+2

storage temp.

room temp

SMILES string

[15N]#[15N]

InChI

1S/N2/c1-2/i1+1,2+1

InChI key

IJGRMHOSHXDMSA-ZDOIIHCHSA-N

Application

Nitrogen-15N2 (15N Labeled nitrogen) can be used:
  • In nitrogen fixation experiments to monitor the resulting metabolites in an organism.
  • 15N2 pulse-chase experiments to understand the route of transport of fixed nitrogen from the nodule to the shoot in plants.
  • To quantify biological N2 production in agricultural soil.

Packaging

250 mL and 500 mL quantities are packaged in a 25 mL Sure/Pac cylinder with brass 1/4" male NPT valve. Nominal gas pressures at 21ºC are 135 psig for 250 mL and 275 psig for 500 mL.

Quantities of 1 L and 5 L are packaged in a 450 mL carbon steel lecture bottle with brass CGA 110/180 valve. Nominal gas pressures at 21ºC are 20 psig for I L and 155 psig for 5 L.

Cylinder pressures are slightly above atmospheric pressure. Care must be taken when extracting this product from the cylinder.
All gas volumes are quoted at Standard Temperature and Pressure (STP) and not at Normal Temperature and Pressure (NTP). One STP Liter is 7.7% more gas than one NTP Liter.
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Other Notes

PTFE sealing washers GCA inlet size 180 Z146994

PTFE sealing washers CGA inlet size 110 Z146986

Mini gas regulator, brass body, appropriate for 250 ML and 500 ML pack sizes Z513539

Non-corrosive lecture-bottle gas regulator, CGA inlet size 180, appropriate for 1L and 5L pack sizes Z146706

Legal Information

Sure/Pac is a trademark of Sigma-Aldrich Co. LLC


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pictograms

Gas cylinder

signalword

Warning

hcodes

Hazard Classifications

Press. Gas Compr. Gas

Storage Class

2A - Gases

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

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Producing and dispensing small quantities of 15N2 gas at atmospheric pressure
Diocares R, et al.
Analytical biochemistry, 352(2), 302-304 (2006)
Min-Hyung Ryu et al.
Nature microbiology, 5(2), 314-330 (2019-12-18)
Legumes obtain nitrogen from air through rhizobia residing in root nodules. Some species of rhizobia can colonize cereals but do not fix nitrogen on them. Disabling native regulation can turn on nitrogenase expression, even in the presence of nitrogenous fertilizer
Quantitative analysis of the initial transport of fixed nitrogen in nodulated soybean plants using 15N as a tracer
Van Phi Hung N, et al.
Soil Science and Plant Nutrition, 59(6), 888-895 (2013)



Global Trade Item Number

SKUGTIN
364584-1L04061831825398
364584-5L-EU04061838204103
364584-500ML04061838204080
364584-5L04061838204097
364584-250ML04061831825411
364584-1L-EU04061831825404