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Merck
CN

600113

L-Arginine-15N4 hydrochloride

98 atom % 15N, 97% (CP)

Synonym(s):

SILAC Amino Acid, (S)-(+)-Arginine hydrochloride-15N4, 15N Labeled arginine hydrochloride

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About This Item

Linear Formula:
H215NC(15NH)15NH(CH2)3C(15NH2)CO2H · HCl
CAS Number:
204633-95-4
Molecular Weight:
214.64
NACRES:
NA.12
PubChem Substance ID:
24881774
UNSPSC Code:
12352209
MDL number:
MFCD00144559
Isotopic purity:
98 atom % 15N
Assay:
≥97% (Chiral Purity), 97% (CP)
Mass shift:
M+4
Form:
solid

Key Documents

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Quality Level

200

mass shift

M+4

InChI

1S/C6H14N4O2.ClH/c7-4(5(11)12)2-1-3-10-6(8)9;/h4H,1-3,7H2,(H,11,12)(H4,8,9,10);1H/t4-;/m0./s1/i7+1,8+1,9+1,10+1;

SMILES string

Cl[H].[15NH2][C@@H](CCC[15NH]C([15NH2])=[15NH])C(O)=O

InChI key

KWTQSFXGGICVPE-JYJSWXFRSA-N

isotopic purity

98 atom % 15N

assay

≥97% (Chiral Purity), 97% (CP)

form

solid

optical activity

[α]20/D +22°, c = 12 in 10% HCl

technique(s)

bio NMR: suitable, protein expression: suitable

mp

226-230 °C (dec.) (lit.)

Related Categories

General description

Arginine is an amino acid with a basic side group. In liver, arginine is cleaved to ornithine and urea. Isotope labeled amino acids are required for the production of isotopically labeled proteins.

Application

L-Arginine-15N4 hydrochloride has been used as a supplement for growing rat hybridoma to generate 15N-labeled monoclonal antibody against GPIHBP1 (glycosylphosphatidylinositol-anchored HDL binding protein 1).

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MBBD7285
MBBD2521
MBBC9925
MBBC6301
MBBB7087V

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Harvey RA, et al.
Biochemistry (Lippincott's illustrated reviews) (2011)
High-resolution imaging of dietary lipids in cells and tissues by NanoSIMS analysis.
Jiang H, et al.
Journal of Lipid Research, 55, 2156-2166 (2014)
Incorporation of radioactive amino acids into proteins of the microsome fraction of guinea-pig liver in a cell-free system.
SIMKIN JL and WORK TS
The Biochemical Journal, 67, 617-624 (1957)
Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteins.
Verardi R, et al.
Advances in Experimental Medicine and Biology, 992, 35-62 (2012)
The incorporation of radioactive amino acids into proteins of the microsome fraction of guinea-pig liver at very short time intervals after administration.
Simkin JL and Sutton CR
The Biochemical Journal, 77, 489-492 (1960)

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