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Merck
CN

608092

L-Isoleucine-13C6,15N

98 atom % 15N, 98 atom % 13C, 95% (CP)

Synonym(s):

SILAC Amino Acid, (2S,3S)-2-Amino-3-methylpentanoic acid-13C6,15N, 13C and 15N Labeled L-isoleucine

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About This Item

Linear Formula:
13C2H513CH(13CH3)13CH(15NH2)13CO2H
CAS Number:
202468-35-7
Molecular Weight:
138.12
NACRES:
NA.12
PubChem Substance ID:
24881989
UNSPSC Code:
12352209
MDL number:
MFCD00144621
Isotopic purity:
98 atom % 13C, 98 atom % 15N
Assay:
95% (CP)
Mass shift:
M+7
Form:
solid

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InChI

1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1/i1+1,2+1,3+1,4+1,5+1,6+1,7+1

SMILES string

[13CH3][13CH]([13CH3])[13CH2][13C@H]([15NH2])[13C](O)=O

InChI key

ROHFNLRQFUQHCH-HUEJSTCGSA-N

isotopic purity

98 atom % 13C, 98 atom % 15N

assay

95% (CP)

form

solid

optical activity

[α]25/D +40.0°, c = 2 in 5 M HCl

technique(s)

bio NMR: suitable, protein expression: suitable

mp

168-170 °C (lit.)

mass shift

M+7

Quality Level

200

Related Categories

General description

L-isoleucine is an essential amino acid that is synthesized from threonine. It is a branched-chain hydrophobic amino acid. L-isoleucine is an isomer of L-leucine.

Application

L-Isoleucine-13C6,15N is used in the immunocapture isotope dilution mass spectrometry (IC-IDMS) to evaluate the suitability of the underlying monoclonal andpolyclonal antibodies (mAbs and pAbs) for new vaccine quantification.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MBBC5832
MBBC8577
MBBC6377
MBBC4788
MBBC3144

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Carrie L Pierce et al.
Vaccine, 35(37), 5011-5018 (2017-08-05)
As a result of recent advances in mass spectrometry-based protein quantitation methods, these techniques are now poised to play a critical role in rapid formulation of pandemic influenza vaccines. Analytical techniques that have been developed and validated on seasonal influenza

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