860336
3,3′,5,5′-Tetramethylbenzidine
≥99%
Synonym(s):
BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
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About This Item
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
Molecular Weight:
240.34
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
EC Number:
259-364-6
Beilstein/REAXYS Number:
2808541
MDL number:
Assay:
≥99%
Form:
powder
Quality Level
assay
≥99%
form
powder
mp
168-171 °C (lit.)
storage temp.
2-8°C
SMILES string
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
General description
3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye. It is a better alternative to syringaldazine for the determination of chlorine in water due to its product stability and no interference from calcium and magnesium ions.
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
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Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Colorimetric method for determination of chlorine with 3, 3?, 5, 5?-tetramethylbenzidine.
Serrat F B
Talanta, 41(12), 2091-2094 (1994)
Iron oxide nanoparticles: Hidden talent
Perez J M
Nature Nanotechnology, 2(9), 535-535 (2007)
Y Jung et al.
Mucosal immunology, 8(4), 930-942 (2015-01-08)
Eosinophils are multifunctional leukocytes that reside in the gastrointestinal (GI) lamina propria, where their basal function remains largely unexplored. In this study, by examining mice with a selective deficiency of systemic eosinophils (by lineage ablation) or GI eosinophils (eotaxin-1/2 double
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 860336-1G | 04061835369980 |
| 860336-5G | 04061835370009 |
| 860336-25G | 04061835369997 |
| 860336-100MG | 04061833033135 |