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Sigma-Aldrich

Orange G

certified by the Biological Stain Commission, Dye content 80 %

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Synonym(s):
1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, Acid Orange 10, Wool Orange 2G
Empirical Formula (Hill Notation):
C16H10N2Na2O7S2
CAS Number:
1936-15-8
Molecular Weight:
452.37
Colour Index Number:
16230
Beilstein:
4120705
EC Number:
217-705-6
MDL number:
MFCD00012457
PubChem Substance ID:
329769438
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, 80%

color

orange to very dark orange, and Red to Very Dark Red and Orange-Red and Red-Orange and Dark Red-Orange

solubility

H2O: 1 mg/mL

λmax

475 nm

ε (extinction coefficient)

≥10000 at 329-331 nm in H2O
≥17000 at 473-479 nm in H2O
≥20500 at 246-250 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;

InChI key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

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Application

Collagen stain for connective tissue. Used with Orcein, Alizarin Blue 2B, and Fast Green FCF in Kornhauser′s quadruple stain for most elementary structures of tissues.

Biochem/physiol Actions

Orange G is used in collagen stain for connective tissue. It is used with Orcein, Alizarin Blue 2B, and Fast Green FCF in Kornhauser′s quadruple stain for most elementary structures of tissues. It is also used along with acid fuchsin and light green in masson trichrome stain for the staining of cellular structures, red blood cells and muscle cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Kierszenbaum AL and Tres L
Histology and Cell Biology: An Introduction to Pathology (2015)
Asli Yuksel et al.
Journal of hazardous materials, 190(1-3), 1058-1062 (2011-03-29)
Complete degradation of azo dye Orange G was studied using a 500 mL continuous flow reactor made of SUS 316 stainless steel. In this system, a titanium reactor wall acted as a cathode and a titanium plate-type electrode was used
Suneet Khandelwal et al.
International journal of oral science, 2(1), 45-52 (2010-08-10)
To analyse the cytomorphological features of keratinocytes in smears obtained from the oral mucosa of tobacco users and from oral squamous cell carcinoma lesions. Oral smears were obtained from clinically, normal appearing mucosa of oral squamous cell carcinoma patients (n=20)
G Thennarasu et al.
Environmental science and pollution research international, 19(7), 2755-2765 (2012-02-23)
The photocatalytic degradation of Orange G (OG) dye has been investigated using synthesised nanocrystalline ZnO as a photocatalyst and sunlight as the irradiation source. The formation of ZnO prepared from its precursor was confirmed through FT-IR and powder X-ray diffraction
Arumugam Sivasamy et al.
Current microbiology, 62(2), 351-357 (2010-07-21)
Biosorption is an eco-friendly and cost-effective method for treating the dye house effluents. Aspergillus niger and Trichoderma sp. were cultivated in bulk and biomasses used as biosorbents for the biosorption of an azo dye Orange G. Batch biosorption studies were
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