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900892

L-Azidohomoalanine hydrochloride

Synonym(s):

AHA

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About This Item

Empirical Formula (Hill Notation):
C4H8N4O2 · HCl
CAS Number:
Molecular Weight:
180.59
MDL number:
UNSPSC Code:
12352209
NACRES:
NA.21
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form

powder or crystals

Quality Segment

reaction suitability

reaction type: click chemistry

mp

139 °C

storage temp.

−20°C

SMILES string

[Cl-].[N+H3][C@@H](CCN=[N+]=[N-])C(=O)O

InChI

1S/C4H8N4O2.ClH/c5-3(4(9)10)1-2-7-8-6;/h3H,1-2,5H2,(H,9,10);1H/t3-;/m0./s1

InChI key

MHHYJRIDKLZZEO-DFWYDOINSA-N

Application

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety. This compound can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. For example the azido-modified protein can be detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS/MS and MALDI-MS analysis.


Pictograms

Flame

Signal Word

Danger

Hazard Codes

Hazard Classifications

Self-react. C

Storage Class

5.2 - Organic peroxides and self-reacting hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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