TissueFab^® bioink -(GelAlgHA)MA Vis/405 nm, low endotoxin formulation is derived from natural polymers – hyaluronic acid, alginate, and gelatin. This ready to print bioink is optimized for 3D bioprinting of tissues and constructs using an extrusion-based 3D bioprinter. TissueFab^® bioink -(GelAlgHA)MA Vis/405 nm, low endotoxin formulation can be used to bioprint cell-laden hydrogels in the desired shape without any supporting material and can be crosslinked in one step using exposure to UV light for further culture and maturation of cells for tissue engineering and regenerative medicine applications.

3D bioprinting is the printing of biocompatible materials, cells, growth factors, and the other supporting materials necessary to yield functional complex living tissues. Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. Hyaluronic acid (HA) is a linear polysaccharide of alternating D-glucuronic acid, and N-acetyl-D-glucosamine found in the extracellular matrix. HA is commonly chemically modified to form covalently crosslinked hydrogels. Alginate methacryloyl also known as AlgMA, is a polysaccharide widely used in tissue engineering obtained from brown algae.

3D bioprinting has been used to generate several different types of tissue such as skin, bone, vascular grafts, and cartilage structures. Based upon the desired properties, different materials and formulations can be used to generate both hard and soft tissues. While several 3D printing methods exist, due to the sensitivity of the materials used, extrusion-based methods with bioinks are most commonly employed.

Low Endotoxin, low bioburden: Endotoxins have been demonstrated negatively impact cellular growth, morphology, differentiation, inflammation and protein expression. Bioburden is defined as the number of contaminated organisms found in a given amount of material. We test each lot for endotoxins as well as total bioburden (aerobic and fungal) to minimize unwanted interactions. For more information: https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/microbiological-testing/pyrogen-testing/what-is-endotoxin

10 mL in HDPE bottle

TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany