Human skin derived collagen type I

Human skin derived type I collagen is a natural biomaterial extracted from nutrient-rich skin tissue, recognized as the most abundant collagen type in the human body. Serving as a major structural matrix protein, it plays a crucial role in skin and various other tissues, including bone, tendon, and fibrous connective tissues. This unique collagen is a heterotrimer composed of one α2(I) chain and two α1(I) chains, arranged in a stable triple helical conformation. Its specific amino acid sequence differentiates it from other collagen types, making it ideal for a variety of biomedical applications.
This collagen effectively facilitates cell attachment due to its recognition by various cell surface receptors present on mammalian cells. The most characterized receptors, integrins α1β1 and α2β1, are expressed by multiple cell types, including mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, chondrocytes, osteoblasts, and lymphocytes. It is characterized by the presence of three distinct regions on SDS-PAGE, corresponding to molecular weights of 100 kDa (Alpha region), 200 kDa (Beta region), and 300 kDa (Gamma region), and contains minimal fragmented proteins, ensuring high purity and functionality.
At a concentration of 3 mg/ml, this material is ideal for 3D hydrogel applications.
This material is isolated from human tissue sourced strictly from American Association of Tissue Banks (AATB) accredited and FDA registered tissue bands and organ procurement organization (OPOs) and strives to meet research needs by providing high quality biomaterials.

This human skin derived type I collagen solution can be used in a variety of biomedical research applications including:


*Enhancing cell attachment, growth, and tissue production for in vitro modeling applications ,
*Creating 3D hydrogels for tissue engineering and regenerative medicine


*Supporting the growth of various cell types including mesenchymal stem cells (MSCs), fibroblasts and endothelial cells


*Servicing as a matrix for wound healing and/or drug delivery systems

Features
*Human-Derived Type I Collagen: Sourced from nutrient-rich skin tissue for high quality and biocompatibility.
*Triple Helical Structure: Facilitates effective cell attachment through recognition by cell surface receptors.
*Versatile Applications: Suitable for coatings, hydrogels, and scaffolds in tissue engineering.

Benefits
*Enhanced Cell Adhesion: Promotes rapid cell attachment and growth for improved experimental outcomes.
*Support for Regenerative Medicine: Provides a reliable matrix for developing functional tissues.
*Customizable Solutions: Adjustable collagen concentration allows for tailored applications based on specific needs.

Collagen-Coated Tissue Cultureware Note: The following are general recommendations. Researcher should optimize parameters based on their specific applications. Optimal collagen concentrations for tissue culture surface coatings may depend on application and cell type and must be determined for each application. Typically, 10 – 100 µg/ml of collagen is used.
1. Dilute collagen with 10-20 mM HCl with a pH of 1.9-2.1 to 0.1 mg/ml. It is recommended to use ultrapure sterile water to make the 10-20 mM HCl.
2. Mix by shaking or vortexing until the solution is homogeneous.
3. Aseptically add collagen solution to tissue culture surface to evenly cover entire surface.
4. Cover and incubate collagen coated surfaces at 37 °C for at least 2 hours. The optimal incubation time may vary depending on the experiment and cell type and should be determined for each application.
5. Transfer collagen coated surfaces to biosafety cabinet and aspirate excess solution from coated surface careful not to scratch the surface. Remove lid to allow for air drying overnight.
6. Rinse the coated culture surface with sterile PBS or culture medium to remove residual acid.
7. Use coated cultureware immediately or keep sterile and store at 2-10 °C. Making 3D Collagen Gels Note: All procedures should be performed in a cold room or on ice. All reagents should be cooled prior to making the hydrogel. To prevent premature gelation, keep solutions between 2-10°C. Concentrations will need to be tested to determine the optimal gel concentration for each application. This material has been tested and found to form a gel at >0.5 mg/ml final concentration. 1. Dilute collagen with 10-20 mM HCl with a pH of 1.9-2.1 to desired concentration. It is recommended to use ultrapure sterile water to make the 10-20 mM HCl. 2. Prepare 10x culture medium or 10x PBS. 3. Combine 9 volumes of chilled collagen solution with 1 volume of chilled 10x concentrated medium or 10x PBS. 4. Gently swirl or pipette the mixture repeatedly. 5. Adjust the pH to 7.0-8.0 with NaOH, preferably around 7.4. Concentration and pH will affect the speed and strength of the gel. Once pH has been adjusted, cells can be added to the solution if desired. 6. Incubate at 37°C for 30-120 minutes for gel formation depending on final concentration.

Purchaser shall not make any attempt to trace or identify donors from whom the HBS material was obtained. Purchaser shall use any data (including genetic information), generated with or by use of the HBS only in compliance with obligations of confidentiality and non-use equivalent with all applicable international and national legislation and guidelines on the protection of personal data and confidentiality, such as EU Directive 95/46 and EU Regulation 2016/679. In addition to the use restrictions contained on the product label and/or product datasheet or other applicable use terms and conditions of sale, Purchaser agrees to use the HBS solely in accordance with the World Medical Association (WMA) Declaration of Helsinki, adopted in 1964 as subsequently amended by WMA on Ethical Principles for Medical Research Involving Human Subjects.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. end-user) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.