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Merck
CN

D199303

2,4-Dinitrophenylhydrazine

reagent grade, 97%, suitable for analytical testing

Synonym(s):

(2,4-dinitrophenyl)-hydrazine, DNPH

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About This Item

Linear Formula:
(O2N)2C6H3NHNH2
CAS Number:
Molecular Weight:
198.14
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352100
MDL number:
Beilstein/REAXYS Number:
615586
Assay:
97%
Grade:
reagent grade
Technique(s):
HPLC: suitable (for analytical testing)
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Product Name

2,4-Dinitrophenylhydrazine, reagent grade, 97%

grade

reagent grade

Quality Segment

assay

97%

contains

33% water (minimum)

technique(s)

HPLC: suitable (for analytical testing)

mp

197-200 °C (lit.)

functional group

hydrazine, nitro

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

General description

2,4-Dinitrophenylhydrazine (DNPH), a hydrazine derivative, is a potential mutagenic agent. Its molecular and crystal structure has been analyzed. The intra- and intermolecular hydrogen bonds in DNPH have been investigated by vibrational spectroscopic analysis. The mechanism of isothermal degradation of DNPH has been studied by differential scanning calorimetry (DSC).

Application

2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls. It may be used as a colorimetric reagent in the quantitative estimation of prednisone.

Packaging

500G contains total wet product weight of ~485G. Variance is due to addition of water to meet DOT requirements.


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Hazard Classifications

Acute Tox. 4 Oral - Desen. Expl. 1

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Storage Class

4.1A - Other explosive hazardous materials

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges



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