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Merck
CN

D199303

2,4-Dinitrophenylhydrazine

reagent grade, 97%, suitable for analytical testing

Synonym(s):

(2,4-dinitrophenyl)-hydrazine, DNPH

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About This Item

Linear Formula:
(O2N)2C6H3NHNH2
CAS Number:
119-26-6
Molecular Weight:
198.14
NACRES:
NA.21
PubChem Substance ID:
24893586
UNSPSC Code:
12352100
MDL number:
MFCD00007578
Beilstein/REAXYS Number:
615586
Grade:
reagent grade
Assay:
97%
Technique(s):
HPLC: suitable (for analytical testing)

Key Documents

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Product Name

2,4-Dinitrophenylhydrazine, reagent grade, 97%

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

grade

reagent grade

assay

97%

contains

33% water (minimum)

technique(s)

HPLC: suitable (for analytical testing)

mp

197-200 °C (lit.)

functional group

hydrazine
nitro

Quality Level

200

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Application

2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls. It may be used as a colorimetric reagent in the quantitative estimation of prednisone.

General description

2,4-Dinitrophenylhydrazine (DNPH), a hydrazine derivative, is a potential mutagenic agent. Its molecular and crystal structure has been analyzed. The intra- and intermolecular hydrogen bonds in DNPH have been investigated by vibrational spectroscopic analysis. The mechanism of isothermal degradation of DNPH has been studied by differential scanning calorimetry (DSC).

Packaging

500G contains total wet product weight of ~485G. Variance is due to addition of water to meet DOT requirements.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

hcodes

H206,H302

Hazard Classifications

Acute Tox. 4 Oral - Desen. Expl. 1

supp_hazards

EUH044

Storage Class

4.1A - Other explosive hazardous materials

wgk

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
0000503037
0000521381
0000521381
0000503037
0000479409

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Kinetic analysis of isothermal decomposition of 2, 4-dinitrophenylhydrazine using differential scanning calorimetry.
Musuc AM, et al.
Thermochimica Acta, 448(2), 130-135 (2006)
Indian J. Chem. B, 32, 986-986 (1993)
2,4-dinitrophenylhydrazine: a sensitive quantitative reagent for determining microgram quantities of prednisone.
A L Woodson
Journal of pharmaceutical sciences, 60(10), 1538-1542 (1971-10-01)
Vibrational spectroscopy and theoretical studies on 2, 4-dinitrophenylhydrazine.
Chis V, et al.
Journal of Molecular Structure, 744, 363-368 (2005)
Chem. Abstr., 118, 22127d-22127d (1993)
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