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F2456

Fluorescein (free acid)

Name: Fluorescein (free acid)
Brand: ALDRICH

Dye content 95%, Powder

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₁₂O₅

CAS Number:

2321-07-5

Molecular Weight:

332.31

UNSPSC Code:

12171500

Colour Index Number:

45350:1

NACRES:

NA.47

PubChem Substance ID:

24894822

EC Number:

219-031-8

MDL number:

MFCD00005050

Beilstein/REAXYS Number:

94324

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Properties

Product Name

Fluorescein (free acid), Dye content 95 %

form

powder

Quality Segment

200

composition

Dye content, 95%

technique(s)

titration: suitable

color

orange to red-brown

mp

320 °C (lit.)

λ_max

496 nm

ε (extinction coefficient)

≥12000 at 282-286 nm, ≥39000 at 236-240 nm, ≥70000 at 488-492 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

InChI key

GNBHRKFJIUUOQI-UHFFFAOYSA-N

Description

General description

Fluorescein (free acid, CI 45350) is a xanthene dye. Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety.

Application

Fluorescein (free acid) has been used as a staining agent in retinal imaging.

Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications. It is also suitable as a marker for the study of motility of bile canaliculi. Some of the clinical applications are evaluating the ocular surface and delineation of brain regions lacking a blood-brain barrier to permit accurate surgical resection of malignant glioma. Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow. It is widely employed as a quantum yield standard.

pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c