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L020000

Sigma-Aldrich

TCA Deblock

Quality Level

type

for DNA synthesis
for DNA synthesis

product line

Proligo Reagents

assay

28-32 g/L (TCA content, titration)

storage condition

dry at room temperature

impurities

≤200 ppm water content (Karl Fischer)

color

clear
clear

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Packaging

4×4 L in glass bottle
450 mL in glass bottle

Preparation Note

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Other Notes

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Signal Word

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

212.0 °F - closed cup

Flash Point(C)

100 °C - closed cup

Certificate of Analysis

Certificate of Origin

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