L030000
ETT Activator
0.25 M
Synonym(s):
5-(Ethylthio)-1H-tetrazole, 5-(Ethylthio)tetrazole solution
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About This Item
Quality Segment
type
for DNA synthesis
product line
Proligo Reagents
storage condition
dry at room temperature
concentration
32-35 g/L (ETT content, titration)
impurities
≤30 ppm water content (Karl Fischer)
color
clear
SMILES string
CCSc1nnn[nH]1
InChI
1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)
InChI key
GONFBOIJNUKKST-UHFFFAOYSA-N
Preparation Note
Acetonitrile / 5-(Ethylthio)-1H-Tetrazole 100 / 3 (v/w)
Other Notes
Activator solution, containing 5-(Ethylthio)-1H- tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2
Storage Class
3 - Flammable liquids
WGK
WGK 3
Flash Point (°F)
35.6 °F
Flash Point (°C)
2.0 °C
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