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About This Item
Form:
liquid
Storage condition:
dry at room temperature
Quality Segment
type
for DNA synthesis
product line
Proligo Reagents
form
liquid
storage condition
dry at room temperature
concentration
70-77 g/L (Activator 42 content, titration)
impurities
≤30 ppm water content (Karl Fischer)
color
clear
SMILES string
FC(F)(F)c1cc(cc(c1)c2n[nH]nn2)C(F)(F)F
InChI
1S/C9H4F6N4/c10-8(11,12)5-1-4(7-16-18-19-17-7)2-6(3-5)9(13,14)15/h1-3H,(H,16,17,18,19)
InChI key
KKJFZIQEWZVVIV-UHFFFAOYSA-N
Preparation Note
Acetronitrile / Activator 42 100 / 7 (v/w)
Other Notes
Activator solution, containing 5-(3,5-bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.
Legal Information
This product and it use is are covered under US Patent Number 7,897,758
Activator 42 is a registered trademark of Sigma-Aldrich Co. LLC
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signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
41.0 °F
flash_point_c
5 °C
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