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L032000

Sigma-Aldrich

DCI Activator

0.25 M

Synonym(s):
DCI activator solution
NACRES:
NA.21

type

for DNA synthesis
for DNA synthesis

product line

Proligo Reagents
Proligo Reagents

assay

29-32 g/L (DCI content, titration)

storage condition

dry at room temperature

impurities

≤30 ppm water content (Karl Fischer)
≤30 ppm water content (Karl Fischer)

color

clear
clear

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Packaging

4×4 L in glass bottle
6×450 mL in glass bottle

Preparation Note

Dicyanoimidazole / Acetonitrile 3/100 (w/v)

Other Notes

Activator solution, containing 4,5-dicyanoimidazole (DCI) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

41.0 °F

Flash Point(C)

5 °C

Certificate of Analysis

Certificate of Origin

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