L040030
Cap A
configured for ABI
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About This Item
NACRES:
NA.21
UNSPSC Code:
12352200
type
for DNA synthesis
product line
Proligo Reagents
form
liquid
shelf life
365 days
concentration
97.0-119.0 g/L (Acetic Anhydride content, titration)
color
clear colorless, to brownish
compatibility
configured for ABI
storage temp.
2-8°C
Quality Level
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General description
Cap A solution contains acetic anhydrideanhydride, lutidine and tetrahydrofuran (THF). It is used after the phosphoramidite reaction/coupling step. The solution is configured for ABI.
Biochem/physiol Actions
Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.
Preparation Note
Tetrahydrofurane / Lutidine / Acetic Anhydride 8/1/1 (v/v/v)
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
supp_hazards
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
6.1 °F
flash_point_c
-14.4 °C
Regulatory Information
危险化学品
易制毒化学品（2类）
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