CN
All Photos(1)

L040150

Sigma-Aldrich

Cap 1

Synonym(s):
Cap A
NACRES:
NA.21

Quality Level

type

for DNA synthesis

product line

Proligo Reagents

assay

conforms (Formulation, GC)

storage condition

dry at room temperature

impurities

≤200 ppm water content (Karl Fischer)

color

clear

Looking for similar products? Visit Product Comparison Guide

Preparation Note

Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)

Other Notes

Cap 1 solution, containing n-methylimidazole, lutidine and acetonitrile (ACN), is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

35.6 °F

Flash Point(C)

2 °C

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service