M6250
2-Mercaptoethanol
≥99.0%
Synonym(s):
β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
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About This Item
Linear Formula:
HSCH2CH2OH
CAS Number:
Molecular Weight:
78.13
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
Assay:
≥99.0%
Bp:
157 °C (lit.)
Vapor pressure:
1 mmHg ( 20 °C)
vapor density
2.69 (vs air)
Quality Segment
vapor pressure
1 mmHg ( 20 °C)
assay
≥99.0%
form
liquid
expl. lim.
18 %
concentration
14.3 M (pure liquid)
refractive index
n
pH
4.5-6 (20 °C, 500 g/L)
bp
157 °C (lit.)
density
1.114 g/mL at 25 °C (lit.)
functional group
hydroxyl, thiol
storage temp.
2-8°C
SMILES string
OCCS
InChI
1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
InChI key
DGVVWUTYPXICAM-UHFFFAOYSA-N
General description
2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.
Application
2-mercaptoethanol is widely used for retarding oxidation of biological compounds in solution.
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
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Flash Point (°F)
165.2 °F - closed cup
Flash Point (°C)
74 °C - closed cup
PPE (personal protective equipment)
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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