Alkali Scientific™ Radiant™ 2X GC-Long Mastermix

RadiantTM GC-Long Hot Start 2x Master Mix has been engineered for the amplification of extremely difficult templates and long amplicons up to 35kb. RadiantTM GC-Long Hot Start 2x Master Mix possesses 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities with an errorrate of 4.55 x 10-7 . The polymerase is ideal for cloning applications, library construction, screening and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM GC-Long Hot Start 2x Master Mix Polymerase is engineered for robust, superior high-fidelity PCR in comparison with standard proof-reading enzymes such as pfu DNA Polymerase. Several point-mutations combined with a new-generation buffer formulation provide for significantly improved processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples.