Alkali Scientific™ BrightStar™ Femto HRP Chemiluminescent Substrate

100pg, 50pg, 25pg, 12.5pg, 6.25pg, 3.13pg Block 5% Milk/TBST 1 hour Incubate in conjugated antibody for 2 hours Goat α-rabbit-HRP 1:10,000 in 5% milk/TBS-T (1/30/14) Wash 3x TBST 10 min Incubate in substrate 5min, drain XS on filter paper Immobilon lot 1331501 exp 08 JUL 2015 Pierce SuperSignal West Fempo lot OG186675 BrightStar™ 2 component lot 14029A/B 1/29/14 Lot 12249 4°C (From 9/7/12 Stability Assay) Luminata Forte Lot 133055 exp 01 MAY 2015 BrightStar™ Lot 14029 1/29/14 single component Expose on cooled CCD camera for 5 minutes at the indicated length of time after addition of the substrate Analyze images on MutiGauge software for relative signal intensities with background correction