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810255P

Avanti

TopFluor® Cholesterol

Avanti Polar Lipids

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Synonym(s):
Bodipy Cholesterol; Bdp-Chol
Empirical Formula (Hill Notation):
C36H51BF2N2O
CAS Number:
878557-19-8
Molecular Weight:
576.61
NACRES:
NA.25

description

23-(dipyrrometheneboron difluoride)-24-norcholesterol

Assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 mg (810255P-1mg)
pkg of 1 × 10 mg (810255P-10mg)
pkg of 1 × 5 mg (810255P-5mg)

manufacturer/tradename

Avanti Polar Lipids

shipped in

dry ice

storage temp.

−20°C

General description

Biomolecules attached to BODIPY, is readily absorbed in the visible light. It results in greater fluorescence quantum yields. It is highly photostable and less sensitive to change in polarity and pH.

Application

TOPFLUOR®Cholesterol is suitable:
  • for cholesterol-uptake assay
  • for BODIPY cholesterol labeling in live cell experiments
  • for the formation of giant Janus liposomes via poly(vinyl alcohol) (PVA)-assisted lipid swelling

Biochem/physiol Actions

BODIPY labelled cholesterol has similar properties to that of the naïve cholesterol. This serves as a cholesterol marker for live cells and aids in trafficking intracellular cholesterol. It might also help in indicating lipid storage diseases.

Packaging

5 mL Amber Glass Screw Cap Vial (810255P-10mg)
5 mL Amber Glass Screw Cap Vial (810255P-1mg)
5 mL Amber Glass Screw Cap Vial (810255P-5mg)

Legal Information

TopFluor is a registered trademark of Avanti Polar Lipids, Inc.

also commonly purchased with this product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

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Maarit Hölttä-Vuori et al.
Traffic (Copenhagen, Denmark), 9(11), 1839-1849 (2008-07-24)
Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. In this study, we employed atomistic simulations and experiments to characterize a cholesterol compound with
James E Shaw et al.
Journal of structural biology, 155(3), 458-469 (2006-08-08)
Elucidating the role that charged membrane proteins play in determining cell membrane structure and dynamics is an area of active study. We have applied in situ correlated atomic force and confocal microscopies to characterize the interaction of the NAP-22 peptide
Florly S Ariola et al.
Biophysical journal, 96(7), 2696-2708 (2009-04-08)
Cholesterol-rich, liquid-ordered (L(o)) domains are believed to be biologically relevant, and yet detailed knowledge about them, especially in live cells under physiological conditions, is elusive. Although these domains have been observed in model membranes, understanding cholesterol-lipid interactions at the molecular
Sandhya Sankaranarayanan et al.
Journal of lipid research, 52(12), 2332-2340 (2011-10-01)
Studies have shown a negative association between cellular cholesterol efflux and coronary artery disease (CAD). Standard protocol for quantitating cholesterol efflux involves labeling cells with [(3)H]cholesterol and measuring release of the labeled sterol. Using [(3)H]cholesterol is not ideal for the
Frederik W Lund et al.
BMC biophysics, 5, 20-20 (2012-10-20)
Cholesterol is an important membrane component, but our knowledge about its transport in cells is sparse. Previous imaging studies using dehydroergosterol (DHE), an intrinsically fluorescent sterol from yeast, have established that vesicular and non-vesicular transport modes contribute to sterol trafficking
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