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Merck
CN

03-0161-00

SMC® Human IL-4 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-4 in human serum and plasma

Synonym(s):

IL-4 Single Molecule Counting Kit, interleukin-4 immunoassay

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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Product Name

SMC® Human IL-4 High Sensitivity Immunoassay Kit, Used for ultrasensitive quantification of Human IL-4 in human serum and plasma

usage

 kit sufficient for 96 wells

species reactivity

human

manufacturer/tradename

SMC®

technique(s)

single molecule counting: suitable

input

plasma (K2 EDTA)
serum

UniProt accession no.

compatibility

for use with FemtoQuest and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

Legal Information

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

Analysis Note

  • Sensitivity: LLOQ 40 fg/mL* (SMCxPRO® Instrument
  • Standard Curve Range: 10-10,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 95% Plasma: 91%
  • Recovery: Serum: 92% Plasma: 96%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Inflammation & Immunology Inflammatory Disease Immune Response Autoimmune Disease

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Biochem/physiol Actions

Although IL-4 was detectable in non-human primate, rat, equine, feline and canine samples, the assay has not been verified for these species.

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

  • Exceptional Sensitivity: The SMC®technology provides outstanding performance in measuring IL-4 levels in human serum and plasma, allowing for accurate quantification.
  • Complete, Ready-to-Use Kit: Includes IL-4 coated beads, detection antibody, standards, buffers, and wash solutions—everything needed for a seamless assay run on the FemtoQuest system.
  • Advance Allergy & Immune Response Studies: IL-4 is central to Th2 cell differentiation, B cell activation, and IgE production. This assay supports research in asthma, atopic dermatitis, and allergic inflammation.
  • Detect Early Immune Shifts: With femtogram-level sensitivity, you can identify subclinical cytokine changes—ideal for early-stage biomarker discovery and therapeutic development.

General description

Interleukin-4 (IL-4) is a cytokine primarily produced by activated T cells, particularly Th2 cells, and plays a crucial role in the immune response. It is essential for the differentiation of B cells, promoting the production of antibodies, and is involved in the regulation of various immune functions, including the enhancement of IgE production, which is significant in allergic responses. Additionally, IL-4 is implicated in the pathogenesis of several diseases, including asthma and other allergic conditions.

Packaging

96 well assay

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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