Skip to Content
Merck
CN

Key Documents

View All Documentation

03-0168-00

SMC® Human IL-5 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-5 in human serum and plasma

Synonym(s):

IL-5 Single Molecule Counting kit, IL-5 immunoassay kit, IL-5 ultrasensitive immunoassay, interleukin-5 immunoassay

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
ELISA: suitable, single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments (validated)
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

SMC® Human IL-5 High Sensitivity Immunoassay Kit,

description

Used for ultrasensitive quantification of Human IL-5 in human serum and plasma

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

ELISA: suitable, single molecule counting: suitable

input

serum
plasma (K2 EDTA)

UniProt accession no.

P05113

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments (validated)

shipped in

wet ice

storage temp.

2-8°C

General description

Interleukin-5 (IL-5) is a cytokine primarily produced by activated T cells, particularly Th2 cells, and plays a pivotal role in the regulation of eosinophil differentiation, activation, and survival. It is essential for the immune response against parasitic infections and is also implicated in allergic reactions and asthma, where elevated levels of IL-5 contribute to eosinophilic inflammation. Understanding IL-5′s functions is crucial for developing targeted therapies for various allergic and inflammatory diseases.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

  • Ultra-High Sensitivity: This IL-5 assay kit offers exceptional sensitivity, allowing researchers to detect low concentrations of IL-5 in biological samples, which is crucial for studying subtle changes in immune responses.
  • Accurate Quantification: With precise measurement capabilities, the kit enables accurate quantification of IL-5 levels, facilitating reliable data collection for research on allergic diseases, asthma, and eosinophilic disorders.
  • Cost-Effective Research Tool: By enabling the early detection of IL-5 and reducing the need for extensive follow-up studies, this high-sensitivity assay kit can help optimize research budgets while providing valuable insights into immune function.
  • SMC Technology Advantage: The incorporation of SMC technology allows for unprecedented sensitivity and accuracy in IL-5 detection, making it a game-changer for researchers aiming to uncover critical insights into immune system dynamics.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 40 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 10-50,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 106% Plasma: 111%
  • Recovery: Serum: 98% Plasma: 100%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IL-5 was detectable in non-human primate, canine, rat, and mouse plasma, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library