03-0171-00
SMC® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit
manufacturer/tradename
SMC®
assay range
sensitivity: 0.04 pg/mL
(lower limit of quantification)
inter-assay cv: <11%
intra-assay cv: <10%
((Intra-assay CV n=20; Inter-assay n=5))
technique(s)
ELISA: suitable
single molecule counting: suitable
relevant disease(s)
Alzheimer′s disease
storage temp.
2-8°C
Related Categories
General description
The SMC® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Specificity
Cross reactivity and interference of ProBDNF was tested in this assay.
The assay shows 1.2% cross reactivity with ProBDNF. Serum and plasma spiked with ProBDNF show an average 4.4% interference.
Accuracy:
- Minimum of 5 EDTA plasma samples spiked with average recovery of 96% with a range of 81-108%
- Minimum of 5 serum samples spiked with average recovery of 90% with a range of 71-98%
- Minimum of 5 cerebrospinal fluid (CSF) samples spiked with average recovery of 98% with a range of 83-108%
Application
- Neuroscience
Research Sub-Category:
- Alzheimer′s Disease
- Neurochemistry
- Neurotrophins
- Signaling Neuroscience
The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of BDNF in human EDTA plasma, serum, and cerebrospinal fluid.
Features and Benefits
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Removal of a plate transfer step
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
Components
- Assay Buffer
- BDNF Coated Beads
- Standard Diluent
- BDNF Detection Antibody
- BDNF Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation
Target Organs
Respiratory Tract
Storage Class Code
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Regulatory Information
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