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Merck
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03-0176-00

SMC® Human IL-10 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-10 in human serum and plasma

Synonym(s):

IL-10 Single Molecule Counting kit, IL-10 immunoassay kit, L-10 ultrasensitive immunoassay, interleukin-10 immunoassay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Key Documents

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product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

manufacturer/tradename

SMC®

technique(s)

single molecule counting: suitable

input

serum

accession no.

P22301

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Interleukin-10 (IL-10) is an anti-inflammatory cytokine primarily produced by regulatory T cells, macrophages, and dendritic cells, playing a crucial role in modulating immune responses. It is essential for inhibiting the production of pro-inflammatory cytokines, thus helping to maintain immune homeostasis and prevent excessive inflammation. IL-10′s regulatory functions make it a key player in various diseases, including autoimmune disorders and chronic inflammatory conditions, highlighting its potential as a therapeutic target.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Biochem/physiol Actions

Although IL-10 was detectable in non-human primate, mouse, rat, canine and feline samples, the assay has not been verified for these species.

Features and Benefits

  • Femtogram-Level Sensitivity for IL-10 Detection: Enables quantification of extremely low levels of IL-10, critical for studying subtle anti-inflammatory responses in immunology and autoimmune disease research.
  • Elution-Based Workflow for Low Background: Reduces background noise and improves signal-to-noise ratio, enhancing assay performance in complex biological samples.
  • Comprehensive Kit for Streamlined Assay Setup: Offers a ready-to-use solution for researchers, minimizing setup time and ensuring consistency across experiments.
  • Supports Autoimmune and Inflammatory Disease Research: Enables quantification of IL-10 in conditions like rheumatoid arthritis, lupus, and psoriasis, where IL-10 plays a dual role in inflammation and immune tolerance.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 100 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 100-100,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 106% Plasma: 106%
  • Recovery: Serum: 87% Plasma 90%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Inflammation & ImmunologyInflammatory DiseaseImmune ResponseAutoimmune Disease

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

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Certificates of Analysis (COA)

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Protocol- 03-0176-00

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