Skip to Content
Merck
CN

03-0181-00

SMC® Human IFN-γ High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IFNɣ in human serum and plasma

Synonym(s):

IFN gamma Single Molecule Counting kit, IFN gamma ultrasensitive immunoassay, interferon gamma immunoassay, interferon immunoassay kit

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

technique(s)

single molecule counting: suitable

input

serum
plasma (K2 EDTA)

UniProt accession no.

P01579

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

Related Categories

General description

Interferon-gamma (IFN-γ) is a key cytokine produced primarily by T cells and natural killer (NK) cells that plays a vital role in the immune response. It is essential for enhancing the immune system′s ability to fight infections, particularly by activating macrophages and promoting the differentiation of T cells into Th1 cells. IFN-γ also has antiviral properties and is involved in the regulation of various immune and inflammatory processes, making it a critical factor in both innate and adaptive immunity.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

  • Ultra-Sensitive Detection of IFN-γ: Enables precise quantification of low-level IFN-γ, critical for studying early immune activation, cytokine signaling, and therapeutic response.
  • Application in Autoimmune and Inflammatory Disease Research: Supports mechanistic studies of inflammation and immune regulation, including diseases like multiple sclerosis, rheumatoid arthritis, and Castleman disease.
  • High Precision and Recovery: Intra-assay CV <15%, inter-assay CV <20%, and recovery rates of 98% in plasma and 104% in serum. Ensures reliable and reproducible data, critical for longitudinal studies, and biomarker validation.
  • Specificity for Human IFN-γ: Provides accurate quantification of IFN-γ, supporting mechanistic studies of Th1 responses, macrophage activation, and MHC expression.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 30 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 20-25,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 96% Plasma: 100%
  • Recovery: Serum: 104% Plasma: 98%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IFN-γ was detectable in non-human primate, mouse, rat, canine and feline samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

Regulatory Information

新产品
This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Power of High Sensitivity Immunology Assays| SMC® Technology

Use the power of high sensitivity immunology assays with SMC® technology to precisely characterize low-abundant cytokines like those from Th1/Th2 and Th17 cells.

Overview of Ultrasensitive Immunoassay Technology | SMC® Technology

Explore ultrasensitive immunoassay technology with SMC® technology, including its biomarker detection platform, advantages of high sensitivity assays, and more.

Protocol- 03-0181-00

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service