Skip to Content
Merck
CN

03-0196-00

SMC® Human Total α-synuclein High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human Total α-Synuclein in human serum, plasma, and cerebrospinal fluid (CSF)

Synonym(s):

Human α-synuclein, α-synuclein, Human total-α-synuclein, a-synuclein, total-a-synuclein

Sign In to View Organizational & Contract Pricing.

Select a Size


About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest and SMCxPRO® instruments
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

technique(s)

single molecule counting: suitable

input

serum
cerebrospinal fluid (CSF)
plasma (K2 EDTA)

UniProt accession no.

compatibility

for use with FemtoQuest and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

General description

α-Synuclein is a small, soluble protein predominantly found in the presynaptic terminals of neurons. It actively participates in synaptic function by regulating neurotransmitter release and maintaining synaptic integrity. Additionally, α-synuclein influences the dynamics of synaptic vesicles and interacts with various proteins involved in synaptic transmission. In neurological disorders, particularly neurodegenerative diseases like Parkinson′s disease, α-synuclein misfolds and aggregates into insoluble fibrils, forming Lewy bodies. These aggregates disrupt cellular function, leading to neuronal toxicity and death. The accumulation of α-synuclein also triggers neuroinflammation by activating microglial cells, which can further contribute to neurodegeneration.

Application

NeuroscienceNeurological DisordersNeurodegenerative DiseasesSynucleinopathies

Features and Benefits

  • Ultra-Sensitive Femtogram Analysis: Achieve exceptionally low detection limits (fg/mL) in serum, plasma and CSF.
  • High-Performance Signal Optimization: Reduced background and expanded dynamic range deliveres highly reproducible results.
  • Intuitive Assay Workflow: Utilize a familiar sandwich immunoassay protocol with next-generation sensitivity.
  • Scalable Automation: Integrate with robotic liquid handling systems to reduce hands on time and improve lab efficiency.
  • Expert Technical Support: Get end-to-end support from field scientists and technical support specialists.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 1,950 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 490-500,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 113% Plasma: 118% CSF: 102%
  • Recovery: Serum: 96% Plasma: 96% CSF: 96%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although Total α-Synuclein was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, and canine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

Regulatory Information

新产品
This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

通过适合的免疫检测产品,探索神经免疫和神经炎症细胞因子及神经退行性疾病生物标志物。

在强大的SMCxPRO®检测平台上,采用单分子计数(SMC®)高灵敏度免疫检测试剂盒,准确可靠地检测健康和病患来源的主要神经学蛋白分析物,进行神经科学研究。分析各种神经科学生物标志物,如神经丝蛋白轻链(NF-L)、胶质纤维酸性蛋白(GFAP)、Tau蛋白、β-淀粉样蛋白等。

Discover how to expand neuroscience biomarker research with high sensitivity immunoassays, such as Single Molecule Counting (SMC®) assays. These assays give researchers the tools they need to detect low-abundant blood-based biomarkers and expand biomarker options in neuroscience research.

Explore high-sensitivity SMC® neurology immunoassays that precisely measure low levels of neurology protein analytes, such as NF-L or Tau proteins.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service